苦参碱对脂多糖／痤疮丙酸杆菌诱导的小鼠肝炎及产生肿瘤坏死因子的影响  

EFFECT OF MATRINE ON MOUSE HEPATITIS AND TUMORNECROSIS FACTOR PRODUCTION INDUCED BY PROPIONIBACTERIUM ACNES/ LIPOPOLYSACCHARIDES

作　　者：胡振林[1] 张俊平[1] 万莫斌 余祥彬[1] 林文[1] 钱定华[1] 

机构地区：[1]第二军医大学药学院中西药研究室,长海医院传染病科

出　　处：《药学学报》1996年第9期662-665,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：用小鼠致死性肝炎模型和ＴＮＦ体外诱生的方法，研究苦参碱（Ｍａｔ）对脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅｓ，ＬＰＳ）诱导的经痤疮丙酸杆菌（ｐｒｏｐｉｏｎｉｂａｃｔｅｒｉｔｔｍａｃｎｅｓ，ＰＡ）预刺激的小鼠产生肿瘤坏死因子（ＴＮＦ）以及致死性肝炎的影响。结果表明：Ｍａｔ（１０，５０ｍｇ·ｋｇ￣（－１），ｉｐ，ｂｉｄ×３ｄ）可降低血清ＴＮＦ和ＡＬＴ水平及小鼠对ＬＰＳ致死毒性的敏感性，并可在体外抑制ＬＰＳ诱导的经ＰＡ预刺激的小鼠腹腔巨噬细胞释放ＴＮＦ。提示Ｍａｔ的保肝作用与其抑制ＴＮＦ释放有关。The effect of matnne (Mat) on lipopolysaccharides (LPS)-induced fatal hepatitisand tumor necrosis factor (TNF) production in Propionibacterium acnes (PA)-pnmed mice werestudied. Mice were injected ip LPS (10 μg/mouse) 7 d after ip PA (0. 5 ml/mouse) to induce fatalhepatitis. Aster ip LPS, serum TNF activity rose to 1657 ± 406 kU. L-1 at 1 . 5 h and ALT activityincreased up to 1496 ± 890 U. L-1 at 5 h. Six of 8 mice died within 5 h and the massive hemorrhagicnecrosis of the liver was observed in all mice. Administration of Mat ( 10, 50 mg.kg -1 , ip, bid × 3 d)before the LPS injection markedly reduced the elevation of serum TNF and ALT activity in a dose-dependent manner, and diminished the mortality induced by LPS. Liver congestion and necrosisinduced by LPS in PA-primed mice were ameliorated markedly by Mat pretreatment. Mat (62 . 5 ￣250mg. L-1) inhibited LPS-induced TNF release from PA-primed mouse peritoneal macrophage in vitroin a concentration-dependent manner. These results seggest that Mat protected PA-primed mice fromthe development of fatal hepatitis induced by LPS due to inhibition of TNF production.

关 键 词：苦参碱 中毒性肝炎 痤疮丙酸杆菌 脂多糖 TNF 

分 类 号：R575.105[医药卫生—消化系统] R285.4[医药卫生—内科学]