人脑挫伤后细胞周期素D1表达的变化  

Changes of Cyclin D1 Expression Following Human Brain Contusion

作　　者：周亦武[1] 肖爱武[2] 邓伟年[2] 孙来晶[2] 舒细记[1] 戴甲培[3] 刘良[2] 徐小虎[1] 

机构地区：[1]汕头大学医学院法医学教研室,广东汕头515031 [2]华中科技大学同济医学院法医学系,湖北武汉430030 [3]华中科技大学同济医学院神经生物学教研室,湖北武汉430030

出　　处：《法医学杂志》2006年第4期245-247,250,F0002,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30570629);中国博士后科学研究基金(2003034459);湖北省自然科学基金(2003ABA140)资助项目

摘　　要：目的研究CyclinD1在人不同部位脑挫伤组织中表达的变化及其与损伤时间的关系。方法88例脑挫伤标本按损伤后存活时间0.5,1,3,24h和3,7,14,30d分为8个实验组,另以6例非脑挫伤的脑作为对照组,应用CyclinD1免疫组织化学并结合图像分析技术观察CyclinD1的变化。结果脑挫伤后,挫伤灶中央CyclinD1阳性细胞几乎丧失,1h后挫伤灶周围CyclinD1阳性细胞开始增加,3h～30d之间各组挫伤灶周围免疫阳性反应的细胞增加显著,在3h～30d一直维持在较高水平;CyclinD1主要见于小胶质细胞和其它胶质细胞,少数神经元也呈阳性。结论人脑挫伤后,CyclinD1在多种脑细胞内表达,以胶质细胞表达明显,CyclinD1阳性细胞在伤后不久即显著增加,故可作为早期脑损伤的诊断指标。Objective To study the relationships of Cyclin D1 expression with the posttraumatic intervals （PTI） following the cerebra, brainstem or cerebella contusion in human. Methods 88 cases of brain contusions of the closed head injury were investigated with pathological and Cyclin D1 immunohistochemistry methods. The results were analyzed by image analysis technique （IAT）. Results The immunoreactivity of Cyclin D1 was almost disappeared in the core cells of the brain contusion. Cyclin D1-positive cells started to increase in the boundary of the brain contusion in the lh group. Cyclin D1-positive cells were increased significantly in the 3 h-30 d groups and maintained at a high level in the boundary of the brain contusion of those groups. It is suggested that the Cyclin Dl-positive cells were primarily origin from microglia and other glia. A few neurons expressed Cyclin D1. Conclusion Cyclin D1 can express in several kinds of brain cells following the contusion, especially in the glia cells. Cyclin D 1-positive cells were increased obviously and rapidly after injury, so it could be used as a reference marker for early stage brain injury.

关 键 词：创伤性脑损伤 脑挫伤 CYCLIN D1 损伤时间 免疫组织化学 图像分析技术 

分 类 号：R651.15[医药卫生—外科学] R737.33[医药卫生—临床医学]