改良AS-PCR引物对ABO基因分型的影响  

Influence of Meliorated AS-PCR Primer for ABO Genotyping

作　　者：王新杰[1] 刁立江 冯建忠[1] 吕桂平[2] 

机构地区：[1]潍坊市公安局,山东潍坊261061 [2]山东省公安厅,山东济南250001

出　　处：《法医学杂志》2006年第4期275-276,共2页Journal of Forensic Medicine

摘　　要：目的通过改进AS-PCR引物,提高ABO基因型分型正确率。方法把引物P13′末端第5位碱基由G改为C,其分型图谱与改进前进行对比分析。结果改进AS-PCR引物后,OO型非特异产物减少,避免了将OO型误判为AO型。结论引物P13′末端第5位碱基由G改为C后,可有效防止OO型被误判为AO型。Objective To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. Methods The primer P1 was changed into primer PI＇ by substituting the fifth base G for C of 3′end and the ABO genotyping results of primer P1 and P1′ was compared and analysed. Results The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. Conclusion The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1′.

关 键 词：AS-PCR 改良引物 3′末端 碱基 ABO基因型 

分 类 号：R457.11[医药卫生—治疗学] R394.8[医药卫生—临床医学]