Bio-Gel P-2分离低聚木糖的特性  

Separation of Xylooligosaccharides on Bio-Gel P-2

作　　者：张军华[1] 勇强[2] 徐勇[2] 余世袁[2] 

机构地区：[1]西北农林科技大学林学院,杨凌712100 [2]南京林业大学化学工程学院,南京210037

出　　处：《食品与发酵工业》2006年第7期111-113,共3页Food and Fermentation Industries

基　　金：国家自然科学基金项目(39770601)

摘　　要：以聚丙烯酰胺凝胶（Bio-Gel P-2）为层析介质，快速蛋白液相层析（FPLC）系统为平台，以脱气超纯水为洗脱液，对其分离低聚木糖的特性进行了探讨。研究表明，Bio-Ge lP-2可用于低聚木耱分离，采用Bio-Gel P-2分离低聚木糖时，木二糖至木六糖各相邻组分之间的选择因子α和分离度R分别介于1．13～1．18和0．83～1．11之间，而且各组分与层析介质Bio-Gel P-2之间无特异性吸附现象，能完全按照分子质量大小进行分离。通过1次分离可获得纯度分别为86．94％、82．90％、88．30％、64．20％和77．89％的木二糖至木六糖溶液。Separation properties of xylooligosaccharides on polyacrylamide gel Bio-Gel P-2 with FPLC system with degassed high purified water as eluant was investigated. The results showed that Bio-Gel P-2 can be used for separation of xylooligosaccharides. The selectivity of Bio-Gel P-2 to xylooligosaccharides is between 1.13 and 1.18. The resolution of the two peaks is between 0.83 and 1.11. There is no particular interaction between xylooligosaccharides and Bio-Gel P-2, and the molecular weight is the dominant factor governing the separation in this system. The purity of xylobiose to xylohexaose separated by once time is 86.94 %, 82.90 %, 88.30 %, 64.20% and 77.89 96, respectively.

关 键 词：低聚木糖 聚丙烯酰胺凝胶 凝胶过滤层析 分离 

分 类 号：TQ202[化学工程—有机化工]