蛋白转导在基因治疗中的应用  

Application of protein transduction in gene therapy

作　　者：熊符[1] 张成[1] 于美娟[1] 

机构地区：[1]中山大学干细胞与组织工程中心,广州510080

出　　处：《国际生物医学工程杂志》2006年第4期250-254,共5页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30170337);教育部博士点基金资助项目(20030558058)

摘　　要：蛋白转导是近几年生命科学领域发现的一种独特现象,具有蛋白转导功能的蛋白通过几个短的碱性小肽组成的蛋白转导域(proteintransductiondomain,PTD)的介导,能将与其共价连接的DNA、多肽或蛋白质以及其他大分子物质通过非经典途径穿过细胞膜甚至血脑屏障,并在细胞间自由传递。PTD这种能携带融合蛋白自由进入细胞的独特功能为人类一些疾病的基因治疗提供了一种新的运载工具,在基因治疗中有着美妙的应用前景。Protein transduction is an emerging technique in biological fields in recent years. Through the medium of protein transduction domain（PTD） composed by a small number of alkaline peptides, the functional proteins linked covalently with DNA, peptides, proteins or other macromolecules can across the membranes of mammalian cells, even the blood-brain barrier and transfer unconventionally that isn＇t like classical pathway. The distinct property of PTD that can take along fused protein entering into cells unconventionally offers a new vehicle to gene therapy for some diseases and has dulcet applied perspective.

关 键 词：蛋白转导 蛋白转导域 基因治疗 

分 类 号：Q789[生物学—分子生物学]