辣椒、番茄中氟虫脲残留量的HPLC分析方法  

HPLC determination of flufenoxuron residues in Capsicum and tomato

作　　者：张清明[1] 花日茂[2] 汤锋[2] 李学德[2] 操海群[2] 岳永德[2] 

机构地区：[1]莱阳农学院植保学院,山东青岛266109 [2]安徽农业大学资源与环境学院,合肥230036

出　　处：《农药》2006年第9期620-621,638,共3页Agrochemicals

摘　　要：建立了测定辣椒和番茄中氟虫脲残留量的高效液相色谱法。色谱柱为ThermoHypersil-ODSC18D250mm×4.6mm;流动相:甲醇∶水(80∶20,v∶v);流速:1.0ml/min;检测波长:254nm。试样用丙酮提取,二氯甲烷萃取,中性氧化铝柱净化,外标法定量。在0.05￣0.5mg/kg的添加浓度下,平均回收率为87.51%￣102.13%,变异系数为0.07%￣6.02%,仪器最低检出限为8.809×10-10g,最小检出浓度0.011mg/kg,该方法的准确度、精密度及灵敏度均满足农药残留分析的要求。An HPLC method for determination of flufenoxuron residues in Capsicum and tomato was established using a Thermo Hypersil-ODS C18 reverse phase column （250 × 4.6 mm）, methanol-water （80 ： 20, by vol） as the mobile phase, a flow rate of 1.0 ml/min, and detection at 245 nm. Capsicum and tomato samples were extracted with acetone, purified using LLE with dichloromethane, cleaned up with a neutral Al2O3 column, and quantitatively analyzed with an external standard method, The average recovery rate was 87.51 to 102.13%, with a coefficient of variation of 0.07 to 6.02%, a limit of detection of 8,809 × 10^-10 g, and a minimum detectable concentration of 0.011 mg/kg. Accuracy, precision, and sensitivity of the method all satisfactorily met the demands of pesticide residue analysis.

关 键 词：氟虫脲 HPLC 辣椒 番茄 残留分析 

分 类 号：TQ450.263[化学工程—农药化工] TS207.53[轻工技术与工程—食品科学]