T细胞受体基因重排检测皮肤 T 细胞淋巴瘤  

Study on Clinical Application of Detection of T-cell Receptor Gene Rearrangement in Cutaneous T-cell Lymphoma

作　　者：张春雷[1] 宫恩聪[2] 刘永生[3] 沈力[1] 李世荫[1] 

机构地区：[1]北京医科大学第三临床医学院皮肤科,100083 [2]北京医科大学第三临床医学院病理学系,导师100083 [3]中日友好医院皮肤科

出　　处：《中华皮肤科杂志》1996年第5期346-348,共3页Chinese Journal of Dermatology

摘　　要：为了研究基因诊断在皮肤 T 细胞淋巴瘤(CTCL)的临床应用,采用聚合酶链反应(PCR)技术,对60例皮肤淋巴细胞浸润疾患进行了 T 细胞受体(TCR)基因重排的检测。结果显示,检出TCR-β和(或)TCR-γ基因克隆重排的有:36/40例 CTCL、4/6例可疑蕈样肉芽肿/Sézary 综合征和1/1例淋巴瘤样丘疹病;未检出 TCR-β或γ基因克隆重排的有:2例皮肤 B 细胞淋巴瘤、1例皮肤淋巴细胞瘤、8例良性皮肤淋巴细胞浸润疾患和2例正常人皮肤。这表明:该方法在确定 CTCL 的克隆性、细胞源性以及早期诊断方面是有帮助的。To study the clinical application of molecular diagnosis in cutaneous T-cell lymphoma (CTCL),the polymerase chain reaction Was used to detect the rearrangements of T-cell receptor (TCR)gene in 60 patients with cutaneous lymphocytic infiltrates.The result showed that dominant clonal TCR-β or γ gene rearrangements were detected in 36/40 CTCL,4/6 suspected mycosis fun- goldes/Sézary syndrome,1/1 lymphomatoid papulosis.No dominant clonal TCR-β or γ gene rearrange- ments were detected in 2 patients with CBCL,1 patient with lymphocytoma cutis,8 patient with benign lymphocytic infiltrates of skin and 2 normal controls.This study demonstrates that the method is help- ful for the lineage determination,clonality and early diagnosis of CTCL.

关 键 词：T细胞淋巴瘤 T细胞受体 聚合酶链反应 皮肤肿瘤 

分 类 号：R739.504[医药卫生—肿瘤]