c-Met抑制剂对人晶状体上皮细胞增殖的抑制作用(英文)  

Inhibition effect of c-Met inhibitor on proliferation of lens epithelial cells

作　　者：安小玲[1] 王维明[2] 韩清[3] 陶津华[1] 张劲松[1] 

机构地区：[1]中国医科大学附属第四医院眼科,中国辽宁省沈阳市110005 [2]黑龙江省庆安县人民医院眼科,中国黑龙江省庆安县152400 [3]哈尔滨医科大学附属第四医院眼科,中国黑龙江省哈尔滨市150001

出　　处：《国际眼科杂志》2006年第4期743-744,共2页International Eye Science

摘　　要：目的:探讨 c-M et抑制剂 K252a 对人晶状体上皮细胞增殖的抑制作用。方法 : 取原代培养人晶状体上皮细胞加入 H G F、H G F+K252a,以 D M EM 为对照,应用四唑盐法(M TT法)观察 K252a 对晶状体上皮细胞生长的抑制作用,蛋白质免疫印迹(W estern blot)方法检测 K252a 对 Bcl-2,C aspase-3 蛋白表达的影响。结果:H G F (50nm ol/L)+ K252a(30nm ol/L)组与对照组光密度值无显著差异 (P>0.05),Bcl-2 和 C aspase-3 的表达水平变化都不明显。结论:c-M et抑制剂 K252a 对人晶状体上皮细胞增殖有抑制作用。AIM： To observe the inhibition of c-Met inhibitor on prolif- eration of lens epithelial cells （LECs）. METHODS： Human＇s LECs were cultured and hepatocyte growth factor （HGF） and K252a were added to second passage of cells supplied with Dulbecco＇s modified eagle＇s medium （DMEM）. MTT assay was used to examine the proliferation of LECs, and Western-blot was used to detect the expression change of Bcl-2 and Caspase-3. RESULTS： The photodensity （A） of HGF （50nmol/L） ＋ K252a （30nmol/L） was not significantly different from that of DMEM control （P〉0.05）. The expression of Bcl-2 and Caspase-3 were not significantly different from that in the control group. CONCLUSION： K252a, the inhibitor of c-Met, can effectively inhibit the proliferation of LECs.

关 键 词：晶状体上皮细胞 C-MET K252A BCL-2 CASPASE-3 

分 类 号：R776[医药卫生—眼科]