霍乱毒素对视神经损伤后视网膜节细胞存活的影响  

Effects of intravitreous injection of cholera toxin on survival of retinal ganglion cells

作　　者：李飞[1] 黄锦桃[1] 李海标[1] 

机构地区：[1]中山大学中山医学院组织学与胚胎学教研室

出　　处：《解剖学杂志》2006年第4期457-459,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金(39870266)

摘　　要：目的：研究霍乱毒素(CTx)对视神经近端损伤后视网膜节细胞存活的影响。方法：视神经近端微挤压断(MC)后,玻璃体内注射CTx,动物存活3、5、7d后采用荧光逆行示踪技术显示存活的视网膜节细胞。结果：在3、5、7d时,移植霍乱毒素组(MC+CTx)存活的视网膜节细胞数分别为(2053±169)、(1881±230)、(1752±137)个/mm^2,明显多于实验对照组(MC)的(1831±158)、(1508±147)和(1240±114)个/mm^2。结论：玻璃体内注入霍乱毒素对视神经微挤压断后视网膜节细胞的存活有明显的促进作用。Objective： To evaluate the effects of cholera toxin （CTx） injected intravhreously on the survival of retinal ganglion cells. Methods： After optic nerve （ON） was microcrushed in adult rats, cholera toxin was injected into vitreous. Animals in each group were allowed to survive for 3, 5, and 7 days, respectively. The survival RGCs were labelled retrogradely with granular blue, and the number of survival RGCs in each retinal were observed under fluorescent microscope. Results： The mean numbers of survival RGCs in cholera toxin group （MC± CTx） were （2 053±169）, （1 881±230）, and （1 752±137）/mm^2 on d 3, d 5 and d 7, respectively, and those in control group （MC） were （1 831±158）, （1 508-147）, （1 240±114）/mm^2, respectively （P〈0. 05）. Conclusion： CTx can significantly enhance the survival of RGCs after optic nerve microcrush.

关 键 词：霍乱毒素 视网膜 节细胞 存活 

分 类 号：R363[医药卫生—病理学]