原代肝细胞培养及其在药物代谢和毒理学研究中的应用进展  

Primary hepatocyte culture and its application in drug metabolism and toxicology research and evaluation

作　　者：邓颖[1] 陈杰[1] 毕惠嫦[1] 黄民[1] 

机构地区：[1]中山大学临床药理研究所,广东广州510080

出　　处：《中国药理学通报》2006年第8期900-903,共4页Chinese Pharmacological Bulletin

基　　金：广州市重大项目(创新药物药理毒理评价研究技术平台;No2003Z2E4071和新药临床前评价研究技术平台;No2004Z1E4051)资助项目

摘　　要：原代肝细胞培养技术已有四十几年的历史,随着技术水平的不断提高,其培养越来越成熟,并且已广泛用于研究药物对细胞色素P450酶的作用及其机制,药物相互作用,药物毒理学等多方面。因为其很好的维持和保留了肝脏的代谢能力,具有与体内一致的细胞色素P450酶的活性,成为日益重要和可信赖的评价药物和新化学实体的安全与有效的体外模型。该文对原代肝细胞培养技术及其在药物代谢和毒理学研究中的应用进展进行了综述。For more than 40 years primary hepatocytes culture technique has been utilized extensively for assessing effects of drug on metabolizing enzymes （especially cytochromes P450）, drug metabolism, drug-drug interactions, and the mechanisms of cytotoxicity and genotoxicity. Human derived primary hepatocytes reserve the metabolism function and enzyme activity of liver. Therefore, this technique has been widely used as a reliable and efficient tool in the drug and xenobiotics evaluation and screen in vitro. This review focuses on primary culture technique of hepatocytes and its application in drug metabolism and toxicology research and evaluation.

关 键 词：原代培养 肝细胞 药物相互作用 药代动力学 CYP450 

分 类 号：R-05[医药卫生] R322.47