染料木黄酮对生长板软骨细胞胶原合成的影响及其机制  

The effect and mechanism of genistein on collagen synthesis of growth plate chondrocytes

作　　者：季煜华[1] 曾耀英[1] 俞瑜[1] 

机构地区：[1]暨南大学组织移植与免疫研究中心教育部重点实验室,广东广州510632

出　　处：《中国药理学通报》2006年第8期927-929,共3页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展规划(973规划)资助项目(NoG1999054303);广东省"十五"重大科技专项资助项目(NoA302020204);暨南大学引进人才启动基金资助项目(No51205066)

摘　　要：目的探讨染料木黄酮(5,7,4′三羟基异黄酮)对大鼠肋生长板软骨细胞(ratcostochondralgrowthplatechondrocyte,RGC)胶原和Sox9表达的影响。方法原代培养RGC,3Hproline参入、RTPCR和Westernblot分别检测染料木黄酮对第1代RGC胶原合成、col2a1基因转录和Sox9表达的影响。结果染料木黄酮抑制RGC的胶原合成(P<0.05),col2a1的转录和Sox9的表达,其抑制作用随着浓度的升高而增强。结论染料木黄酮抑制培养RGC胶原合成的作用至少部分是通过下调Sox9的表达实现的。Aim To investigate effects of genistein （5, 7, 4＇-trihydroxyisoflavone） on rat costochondral growth plate chondrocyte （RGC） collagen and Sox9 expression. Methods Primary cultured RGC, effects of genistein on collagen synthesis, col2al mRNA transcription and Sox9 protein expression of 1st passage RGC were detected by isotope incorporation, RT-PCR and western blotting. Results Genistein inhibited collagen synthesis of 1st passage RGC （P 〈 0.05 ）,col2al mRNA transcription and Sox9 protein expression. The inhibitory effect of genistein enhanced with its increasing concentration. Conclusion The inhibitory effect of genistein on collagen synthesis of RGC is partially mediated by the down-regulation of Sox9 expression.

关 键 词：染料木黄酮 生长板软骨细胞 Ⅱ型胶原 SOX9 

分 类 号：R-322[医药卫生] R282.71