N-V蛋白酶体内外纤维蛋白溶解活性的实验研究  

The experimental study on fibrinolytic activity of N-V proteinase in vivo and vitro

作　　者：李奇[1] 迟秀梅[1] 石立红[1] 王艳萍[1] 洪敏[1] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学教研室,吉林长春130021

出　　处：《中国药理学通报》2006年第8期976-979,共4页Chinese Pharmacological Bulletin

基　　金：吉林省科技厅发展资助项目(2004;科技字;No0040);吉林省计委资助项目(2004;科技字;No0710)

摘　　要：目的研究NV蛋白酶对纤溶系统的影响。方法采用纤维蛋白平板法,观察NV蛋白酶体外溶解纤维蛋白的活性。通过一次静脉给药后不同时间测定家兔纤维蛋白原(Fg)、D二聚体(DDimer)的含量及血浆优球蛋白溶解时间(ELT),观察NV蛋白酶对正常家兔纤维蛋白溶解活性的影响。通过测定家兔凝血酶原时间(PT)、活化部分凝血活酶时间(APTT),观察NV蛋白酶对凝血功能的作用。结果NV蛋白酶具有体外溶解纤维蛋白的活性,测定效价为30000kU·g-1。NV蛋白酶(6000U·kg-1体重,15000U·kg-1体重)可明显减少DDimer、Fg含量,缩短ELT,对PT、APTT无明显影响。结论NV蛋白酶具有纤维蛋白溶解活性,对凝血功能未见有影响。Aim To study the influence of N-V proteinase on fibrinolytic system. Methods The fibrin plate method was used to study the hydrolyzing fibrin activity of N-V proteinase in vitro. The content of Fg and D-Dimer, and ELT were assayed to study the effect of N-V proteinase on activity of fibrinolysis in normal rabbits. PT, APTY were measured to observe the effect of N-V proteinase on blood coagulation function in vivo. Results N-V proteinase possessed the fibrinolytic activity in vitro. The titer was 30 000 U·mg^-1 N-V proteinase （ 6 000 U · kg^-1, 15 000 U · kg^-1 ）. It obviously decreased the content of Fg and D- Dimer, and lessened ELT, while had not influence the APTY and PT. Conclusion N-V proteinase possesses the fibrinolysis activity, and the effect on blood coagulation function are not observed.

关 键 词：N—V蛋白酶 纤溶活性 凝血功能 

分 类 号：R-322[医药卫生] R345.63