吸烟对牙龈上皮细胞凋亡的影响  

Influence of smoking on apoptosis in human gingival epithelium

作　　者：于西佼[1] 李纾[1] 薛兰德[2] 肖长杰[1] 

机构地区：[1]山东大学口腔医学院牙周病科,山东济南250012 [2]济南市口腔医院特诊科,山东济南250012

出　　处：《上海口腔医学》2006年第4期351-355,共5页Shanghai Journal of Stomatology

基　　金：山东省优秀中青年科学家奖励基金(02BS096)

摘　　要：目的:观察不同程度吸烟患者的牙龈上皮棘层和基底层细胞凋亡的情况,评价吸烟对牙龈上皮细胞正常代谢和蛋白质分泌的影响。方法:选取47例具有不同吸烟史、无严重系统疾病且行牙冠延长术或智牙拔除术的患者为研究对象,男41例,女6例,年龄18～29岁(平均年龄21.4岁),根据吸烟史、日平均吸烟支数和总吸烟支数,将患者分为Ⅰ(轻)度(吸烟史<5a,或2≤日均支数<10且总支数约1.5万支以下)19例,Ⅱ(重)度(5a≤吸烟史,或10≤日均支数且总支数约1.5万支以上)28例。另取无吸烟史的10例患者作为对照组。采用原位末端标记(TdT-mediated-dUTPnickendlabeling,TUNEL)法检测牙龈上皮棘层和基底层细胞的凋亡情况。采用Newman-Keuls法比较各吸烟组之间及其与对照组间的差异,取α=0.05水准。结果:吸烟者的牙龈上皮棘层和基底层细胞凋亡阳性的表达高于未吸烟者,且棘层(P<0.01)改变较基底层(P<0.05)明显。轻度吸烟组和重度吸烟组之间也存在显著性差异(P<0.05、P<0.01)。结论:吸烟对牙龈上皮棘层的凋亡具有明显的促进作用,从而干预上皮细胞的正常蛋白分泌和代谢。PURPOSE： Cigarette smoking was a major risk factor for oral hygiene and periodontal status. The study was to investigate the relationship between different exposure to cigarette smoke and apoptosis in stratum spinosum and stratum basale cells of human gingival epithelium. METHODS： 47 smoking patients with crown lengthening surgery or extraction of the impacted teeth were enrolled. It comprised 41 males and 6 females. Their age ranged from 18 to 29 years old （average age：21.4）. According to smoke history （SH）, average cigarettes per day （ACD） and total smoke number （TSM）, 47 smokers were divided into group Ⅰ （SH〈5a or ACD〈10 and TSM〈15 thousand,n=19）, group Ⅱ （5a≤SH or 10≤ACD and 15 thousand ≤ TSM,n=28）. Another 10 non-smoking patients were chosen as control. Apoptosis was identified by the terminal deoxy-transferase （TdT）-mediated dUTP-biotin nick end labeling （TUNEL） method. Differences between the two groups were analyzed using Student-Newman-Keuls （SNK）.The criteria for statistical significance was accepted at the probability level P〈0.05. RESULTS： There was increased apoptosis in stratum spinosumin and stratum basale cells of smokers compared to that of non-smokers. Apoptosis in stratum spinosumin cells was significantly different between smokers and non-smokers （P〈0.01）, between group Ⅰ and group Ⅱ （P〈0.01）. CONCLUSION： It is conclude that exposure to cigarette smoke can increase apoptosis in stratum spinosumin cells of human gingival epithelium, which may influence its normal metabolism and protein secretion. Supported by Award Fund for Excellent Young and Middle-Aged Scientists of Shandong Province （Grant No.02BS096）.

关 键 词：吸烟 细胞凋亡 原位细胞凋亡检测 牙龈上皮 

分 类 号：R780.1[医药卫生—口腔医学]