三七皂苷Rg1对t-PA和PAI-1水平的影响  

Effects of ginsenoside-Rg1 on levels of t-PA and PAI-1

作　　者：詹合琴[1] 李平法[2] 杨锦南[1] 莫景花 沈志强[4] 

机构地区：[1]新乡医学院药理教研室,河南新乡453003 [2]新乡医学院检验系,河南新乡453003 [3]新乡市卫生学校,河南新乡453000 [4]昆明医学院云南天然药物药理重点实验室,云南昆明650031

出　　处：《中国药理学通报》2006年第7期869-872,共4页Chinese Pharmacological Bulletin

基　　金：河南省教育厅自然科学基础研究计划资助项目(No2006310013)

摘　　要：目的探讨三七皂苷Rg1对组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物(PAI-1)水平的影响及其作用机制。方法运用发色底物法测定三七皂苷-Rg1在家兔体内、外对血浆纤溶酶原激活物(t-PA)和血浆或血小板释放的纤溶酶原激活物抑制物(PAI-1)水平的影响。结果三七皂苷-Rg1在体外能抑制血浆PAI-1活性,提高血浆t-PA活性,其作用强度呈现出剂量依赖性;静脉内给药显示:三七皂苷-Rg1 30、60、120和240 mg.kg-1组能降低血浆或血小板释放的纤溶酶原激活物抑制物(PAI-1)水平,增强血浆t-PA活性,而且本品还能降低激活的血小板所释放的PAI-1水平。结论三七皂苷Rg1能够有效对抗由于PAI-1活性增高和t-PA活性降低所引起的血栓,这可能是其具有抗血栓作用的有效机制之一。Aim To explore the effects and its mechanisms of ginsenoside-Rg1 on level of t-PA and PAI-1. Methods Type 1 plasminogen activator inhibitor （PAI-1） and tissue type plasminogen activator （t-PA） activity in plasma were assayed using chromogenic substrate. Results The results showed that ginsenoside- Rg1 in vitro or in vivo significantly inhibited PAI- 1 activity, while increased t-PA activity. These effects were concentration-dependent. Intravenous Panax notoginsenoside Rgl at 30,60, 120 and 240 mg ·kg^-1 markedly suppressed PAI-1 level in plasma as well as platelet-released substances stimulated by thrombin, while increased plasma t-PA activity. And release level of PAI-1 owing to blood platelet was greatly decreased by ginsenoside-Rgl. Conclusion Ginsenoside-Rgl showed potent antithrombosis due to the inhibition of PAI-1 and increase of t-PA. It might also be a advantagous mechanism to its antithrombsis.

关 键 词：三七皂苷RG1 抗血栓 纤溶酶原激活物 纤溶酶原激活物抑制物 

分 类 号：R-332[医药卫生] R284.71