薯蓣皂甙元诱导人卵巢癌细胞系HO-8910凋亡及对Survivin基因表达的影响  

The influence of diosgenin on the induction of apoptosis and the expression of survivin in human ovarian cancer HO-8910 cell line

作　　者：苏赞妍[1] 霍木兰[1] 

机构地区：[1]广东省湛江中心人民医院妇产科,广东湛江524037

出　　处：《中国医师杂志》2006年第9期1161-1163,共3页Journal of Chinese Physician

摘　　要：目的观察薯蓣皂甙元对人卵巢癌细胞系HO-8910凋亡的诱导作用,并探讨其凋亡机制与survivin基因的关系。方法通过MTT法,流式细胞仪等方法研究薯蓣皂甙元对人卵巢癌细胞系HO-8910的诱导凋亡作用,运用RT-PCR分析薯蓣皂甙元对HO-8910细胞survivin表达的影响。结果薯蓣皂甙元在浓度为12.5μg/m l至100μg/m l,作用HO-8910细胞24、48、72 h可以明显抑制HO-8910细胞增殖,与对照组相比差异具有统计学意义(P<0.05),且呈时间剂量依赖性。在浓度为25μg/m l及50μg/m l时,流式细胞仪可以检测到明显的凋亡峰,且HO-8910细胞表达Survivin基因较用药前明显下降。结论薯蓣皂甙元可以诱导HO-8910细胞凋亡,其机制可能与抑制survivin表达有关。Objective To investigate the effects of diosgenin on induction of apoptotis and the relationship between the apoptotic process and the expression of survivin in human ovarian cancer HO-8910 cell line. Methods Methylthiazolyl tetrazolim （MTT） assay and flow cytometry （FCM） were used to study the effects of diosgenin on the induction of apoptosis in HO-8910 cell line. The influence of diosgenin on the expression of survivin in HO-8910 was evaluated by RT-PCR. Results The cell proliferation was significantly inhibited when the HO-8910 cells were treated with diosgenin at the concentration of 12.5 μg/ml to 100 μg/ml for 24, 48, and 72 h, respectively. The apoptotis rates of HO-8910 were different with different time of treatment. The apoptotic peak was detected by using FCM when the cells were treated with 25 μg /ml and 50 μg/ml diosgenin. The expression of survivin was decreased in HO-8910 cells with the treatment of diosgenin. Conclusion Diosgenin may induce the apoptosis of HO-8910 cell line, and the inhibition of survivin expression may involved in the mechanism.

关 键 词：薯蓣皂甙 卵巢肿瘤 细胞凋亡 基因表达 

分 类 号：R737.31[医药卫生—肿瘤]