Survivin和P53在反应性和肿瘤性星形胶质细胞中的表达及意义  

Expression and significance of Survivin and P53 in reactive and neoplastic astrocytes

作　　者：刘凤玉[1] 董燕燕[2] 王爱春[2] 张春庆[2] 李丹阳[2] 王丹丹[2] 戚基萍[2] 

机构地区：[1]哈尔滨医科大学科研处,黑龙江哈尔滨150081 [2]哈尔滨医科大学第一临床医学院病理科,150001

出　　处：《哈尔滨医科大学学报》2006年第4期275-278,共4页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(30470661)

摘　　要：目的检测Survivin和P53蛋白在星形胶质细胞反应性增生与星形胶质细胞瘤中的表达，探讨二者在胶质瘤形成和发展中的作用及意义。方法应用组织芯片和免疫组化染色技术检测正常脑组织、星形胶质细胞反应性增生、低级别（Ⅰ～Ⅱ级）和高级别（Ⅲ～Ⅳ级）星形胶质细胞瘤中Survivin和P53蛋白的表达情况。结果正常脑组织中Survivin和P53蛋白均呈阴性表达；反应性增生组、低级别肿瘤组及高级别肿瘤组中Survivin蛋白的阳性率分别为26、7％（12／45）、53．2％（25／47）、88．1％（37，42）；P53蛋白的阳性率分别为23．8％（10，41）、53．7％（22，41）、79．5％（35／44）。二者阳性表达率均随着病变程度的加重而升高，Survivin和P53在各实验组间比较均差异显著（P〈0．05）；且Survivin和P53表达密切相关（P〈0．01）。结论Survivin和P53在星形胶质细胞反应性增生及星形胶质细胞瘤中均呈不同程度的过度表达，且随着恶性程度的增加其表达水平增高；Survivin扩增和P53突变是胶质瘤发生的早期事件，二者的联合检测可能会为星形胶质细胞反应性增生与低级别胶质细胞瘤的鉴别诊断以及星形胶质细胞瘤的早期诊断提供一定的依据。Objective To study the expression of Survivin and p53 gene products in reactive and neoplastic astrocytoma and analysie the relationship with the tumorgenesis and progression of human astrocytomas. Methods Immunohistochemistry and tissue microarray techniques were used to determine the expression of Survivin and P53 protein in 49 cases of astrocytes proliferation,49 cases of low-grade astrocytomas, and 50 cases of highgrade astrocytomas, 12 cases of normal brain tissues were used as controls. Results In 12 cases of normal brain tissues, the staining were all negative. In reactive astrocytes, low-grade astrocytomas and high-grade astrocytomas, the positive staining of Survivin protein were 26.7%, 53.2%, 88.1%, respectively, and the positive staining of P53 protein were 23.8%, 53.7%, 79.5%, respectively. Among the three experimental groups, there were all statisticaly significant in expressions of Survivin and P53（ P 〈 0.05）. The expression of Survivin was positively associated with P53 protein （P 〈 0.01）. Conclusion ①Survivin and P53 protein are overexpressed in reactive astrocytes and astrocytomas to a certain extent, and the positive rate of expression gradually improves with the histological grade becoming more serious. ②The overexpression of Survivin and the inactivation of P53 might be the early event in the tumorgenesis and progression of human astrocytomas. Survivin and P53 might be combined utilized to discriminate between reactive astrocytes proliferation and low-grade astrocytomas and to provide evidences for earlier diagnosis of astrocytoma.

关 键 词：组织芯片 星形胶质细胞反应性增生 星形胶质细胞瘤 SURVIVIN P53 

分 类 号：R739.41[医药卫生—肿瘤]