Tumstatin治疗喉鳞癌的实验研究  

Effect of tumstatin on laryngeal squamous cell carcinoma

作　　者：肖辉[1] 金德均[1] 王超[1] 徐秀玉[2] 杨静波 刘兴汉[3] 

机构地区：[1]哈尔滨医科大学第二临床医学院耳鼻咽喉科,黑龙江哈尔滨150081 [2]哈尔滨医科大学第三临床医学院,黑龙江哈尔滨150040 [3]哈尔滨医科大学生物化学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2006年第4期294-297,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅资助项目(10551127)

摘　　要：目的探讨肿瘤抑素(Tumstatin)对喉鳞癌细胞(Hep-2)的治疗作用。方法用Hep-2细胞株进行传代培养,台盼蓝法观察Tumstatin和顺铂对不同培养时间细胞的生长抑制情况;MTT法检测不同浓度的Tumstatin对Hep-2细胞存活率的影响,光镜观察细胞形态的变化;电镜和3′-原位末端标记法(TUNEL)检测Hep-2细胞凋亡的发生和形态学改变。结果①随着药物对Hep-2细胞作用时间的延长,细胞生存率明显降低,存在时间依赖性;②MTT检测显示,Tumstatin对细胞的增殖的抑制效应具有剂量依赖性;③光镜观察发现,药物作用组细胞出现病变;④电镜和TUNEL染色证实,Tumstatin对Hep-2细胞的抑制作用是以促进细胞凋亡为主。结论Tumstatin可能通过促进细胞凋亡发挥抗肿瘤的作用。Objective To study the effect of tumstatin on laryngeal carcinoma strain. Methods The laryngeal squamous cell carcinoma Hep-2 strain was chosen in this experiment. The inhibitory effects of tumstatin and cisplatin（DDP） on Hep-2 strain were assayed with MTT test and recorded the changes of cultured cells. The form of Hep-2 cells was observed by electronic microscope and the apoptosis was detected by TUNEL test. Resuits ①After the administration of tumstatin and DDP, the survival rate of Hep-2 ceils decreased significantly with time-dependent. ②Tumstatin and DDP inhibited the proliferation of Hep-2 cells with dose-dependent.③ The ceils of tumstatin and DDP group had pathological changes. ④The fact that apoptosis was a major way of Hep-2 cell death after drug treatment were confirmed by TUNEL test and electronic microscope. Conclusion Tumstatin and DDP may suppress carcinoma by accelerating apoptosis.

关 键 词：TUMSTATIN 喉鳞癌细胞 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]