大鼠放射性口腔黏膜炎模型的建立  

Establishment of murine model of radiation-induced oral mucositis

作　　者：冯崇锦[1] 郭俊兵[1] 李楚玉[2] 王汉渝[2] 李春阳[3] 曹新平[2] 程斌[3] 

机构地区：[1]中山大学附属第一医院口腔科,广州510080 [2]中山大学附属肿瘤医院放疗科,广州510080 [3]中山大学附属口腔医院,广州510080

出　　处：《中华放射医学与防护杂志》2006年第4期346-348,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：广东省科技计划基金资助项目(2004B34001003)

摘　　要：目的建立放射性口腔黏膜炎动物模型。方法70只SD大鼠左侧颊黏膜接受X射线照射,共8次,总吸收剂量为80Gy。照射20、40、60和80Gy后,以及80Gy照射结束后2、4、6、8、14和21d随机处死6只,取左侧颊黏膜进行病理组织学检查,右侧颊黏膜作为自身对照。结果当总吸收剂量达到60Gy时左侧颊黏膜开始出现肉眼可见的红斑,照射80Gy后开始出现单个或多个溃疡,80Gy照射结束后4d左右出现大面积溃疡,80Gy照射结束后2周左右溃疡基本愈合。结论该模型制作方法能较好地模拟大鼠放射性口腔黏膜炎的发病过程,可用于放射性口腔黏膜炎的实验研究。Objective To establish a murine model of radiation-induced oral mucositis. Methods Left-sided buccal mucosa of 70 Sprague-Dawley rats were irradiated with X-rays （ 10 Gy/d）, then six rats, selected randomly, were sacrificed at the 2nd, 4th, 6th, 8th, 14th, and 21st day after the irradiation, left-sided buccal mucosa were excised, corresponding irradiation dose for the selected rats were 20, 40, 60 and 80 Gy following radiation. The right buccal mucosa was excised and treated as its auto-controls tissue. Results Erythema was observed in the left-sided buccal mucosa in rates irradiated with X-rays of 60 Gy; a single or more ulcers observed in rates irradiated with 80 Gy X-rays; a large area of ulcer was observed at 4th day after 80 Gy irradiation. The radiation-induced ulcer in buccal mucosa was essentially recovered after about 2 weeks following 80 Gy irradiation. Conclusion This murine model of radiation-induced oral mucositis was useful and practical in experimental studies.

关 键 词：口腔黏膜炎 放射性 动物模型 大鼠 

分 类 号：R-332[医药卫生]