二硝基氯苯引起大鼠结肠炎与神经源性肠炎的关系  

Dinitrochlorobenzene-induced colitis and its correlations with neurogenic inflammation of gut in rats

作　　者：吴星宇[1] 潘慧[1] 黄鹏[1] 吴彬[1] 蒋惠君[2] 梅林[1] 

机构地区：[1]北京大学医学部生理学与病理生理学系,北京市100083 [2]北京大学医学部病理学系,北京市100083

出　　处：《世界华人消化杂志》2006年第21期2067-2072,共6页World Chinese Journal of Digestology

基　　金：北京市自然科学基金;No.7063083~~

摘　　要：目的：以2,4-二硝基氯苯(DNCB)致大鼠结肠炎,探索免疫因素的作用以及结肠炎与肠神经系统的关系．方法：给♂SD大鼠以DNCB涂腹致敏,然后以DNCB灌肠或脊髓珠网膜下腔注射(ith)激发过敏．DNCB灌肠或ith注射后,根据每日记录的动物体质量和大便性状做疾病活动指数(DAI)评分：d 10-15处死动物后,做结肠大体评分、结肠HE染色的镜下评分以及肠壁组织白细胞移动抑制因子(LMIF)的免疫荧光测定．结果：4g/L和8g/L DNCB灌肠组大鼠的结肠大体和镜下评分明显高于600mL/L乙醇灌肠组(大体评分：2．200±0．416,3．857±0．143 vs 0．143±0．143,P＜0．05：镜下评分：2．000±0．471,3．714±0．184 vs 0．429±0．297,P＜0．05),其肠壁组织中LMIF的荧光染色也明显强于乙醇组．ith DNCB 8和16g/L组大鼠的DAI评分明显高于ith 600 mL/L乙醇组(P＜0．05和0．01),ith 4,8和16g/L DNCB大鼠的结肠组织镜下表现为黏膜层水肿、大量炎性细胞浸润、黏膜糜烂坏死和溃疡形成．结论：DNCB灌肠或ith注射可使致敏大鼠出现结肠炎,肠神经系统和神经免疫介导物LMIF在DNCB所致实验性结肠炎中起重要作用．AIM： To explore the immune mechanism of ulcerative colitis as well as the relationship between ulcerative colitis and enteric nervous system. METHODS： Male Sprague Dawley rats were divided into 2, 4-dinitrochlorobenzene （DNCB） groups and ethanol （control） group. After sensitized by DNCB smeared on the abdominal skin, the rats were challenged with DNCB by enema or intrathecal injection. The weight, stool viscosity and hematochezia were observed and accumulated as the disease active index （DAI） score; the colon pathological score was achieved by macropathology and HE staining of section prepared for microscopy; and the activity of leukocyte migration inhibitory factor （LMIF） was determined by immunofluorescence staining in colon tissues. RESULTS： In the rats treated with 4 and 8 g/L DNCB enema, the macropathological and microscopic scores were significantly higher than those in the controls （macropathological： 2.200 ± 0.416, 3.857 ± 0.143 vs 0.143 ± 0.143, P 〈 0.05; microscopic： 2.000 ± 0.471, 3.714 ± 0.184 vs 0.429 ± 0.297, P 〈 0.05）. The colon tissue showed higher fluorescence intensity of LMIF in the rats treated with DNCB enema than that in the controls. In the rats intrathecally injected with 8 and 16 g/L DNCB, the DAI scores were significantly higher than those in the control rats （P 〈 0.05 and P 〈0.01）. Colon HE staining showed mucosal edema in the rats intrathecally injected 4 g/L DNCB, infiltration of numerous inflammatory cells in those with 8 g/L DNCB, and mucosal erosion, necrosis, and ulceration in those with 16 g/L DNCB. CONCLUSION： Colitis can be induced by DNCB enema or intrathecal injection in sensitized rat, which reflects a delayed type of hypersensitivity （DTH）. The enteric nervous system and neuroimmune mediator LMIF play important roles in the DNCB-induced colitis.

关 键 词：结肠炎 神经源性炎症 2 4-二硝基氯苯 大鼠 

分 类 号：R574.62[医药卫生—消化系统] R285.5[医药卫生—内科学]