持续静压力对人牙周膜细胞RANKL mRNA表达的影响  

Effect of continuously compressive pressure on the expression of RANKL mRNA in human periodontal ligament cells in vitro

作　　者：黄生高[1] 张建兴[1] 熊培颖[1] 王明朗[1] 

机构地区：[1]中南大学湘雅二医院口腔科,长沙410011

出　　处：《中南大学学报（医学版）》2006年第4期518-522,共5页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(03JJY4033)

摘　　要：目的:研究在持续静压力(continuously compressive pressure,CCP)作用下人牙周膜细胞(hu-man perio donta lligament cells,HPDLCs)核因子КB受体活化因子配体(receptor activator of nuclearfactor kappaB ligand,RANKL)的表达变化,探讨其在正畸性骨改建中的作用机制。方法:用组织块酶消化法原代培养HPDLCs,建立压力模型,分别对细胞施以1,2,3g/cm2顶-底轴向压力0.5,1.5,6,12,24和48h,利用逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)法检测RANKLmRNA的表达变化。结果:随着CCP作用时间的增加,HPDLCs RANKL表达增强(P<0.01);随力值增加,RANKL mRNA表达增强,力值为2g/cm2时,改变最明显(P<0.05)。结论:CCP可上调HPDLCsRANKLmRNA表达。Objectiv the expression of receptor ligament cells （HPDLCs） e To determine the effect of continuously compressive pressure （CCP） on activator of nuclear factor kappa B ligand （RANKL） in human periodontal and to investigate the role of RANKL in alveolar bone rebuilding during orthodontic tooth movement. Methods The primary HPDLCs were isolated from human periodontal lig- ament by explanting enzymatic digestion with trypsin and collagenase to establish a pressure model. Top-bottom axial pressures （ 1 , 2, and 3 g/cm^2 ） were laid on HPDLCs for 0.5, 1.5, 6, 12, 24, and 48 h, respectively. The RANKL expression was identified by the reverse transcription-polymerase chain reaction （ RT-PCR ） at the mRNA level. Results The expression of RANKL mRNA significantly increased in a time-dependent manner （ P 〈 0.01 ） , so did the value of pressure, especially in the 2 g/cm^2 group （ P 〈 0. 05 ）. Conclusion CCP can up-regulate the expression of RANKL mRNA in human periodontal ligament cells.

关 键 词：压力 牙周膜细胞 细胞核因子KB受体活化因子配体 逆转录聚合酶链反应 

分 类 号：R783.5[医药卫生—口腔医学]