内源性硫化氢对大鼠血小板精氨酸转运的影响  

Effect of Endogenous H_2S on Platelet L-Arg Transport

作　　者：王一鹏[1] 李颖[2] 耿彬[3] 段文卓[1] 刘同美[1] 唐朝枢[3] 

机构地区：[1]潍坊医学院病理生理学教研室,山东潍坊261042 [2]北京妇产医院生殖遗传室 [3]北京大学第一医院心血管研究所

出　　处：《潍坊医学院学报》2006年第4期258-260,i0002,共4页Acta Academiae Medicinae Weifang

基　　金：国家自然科学基金(30400151)

摘　　要：目的观察新型内源性气体信号分子硫化氢(H2S)对血小板L-精氨酸(L-Arg)转运的影响以探讨硫化氢对血小板功能的影响。方法本试验采用饱和H2S溶液作为H2S供体,制备大鼠富血小板血浆,使用含有不同浓度硫化氢的孵育液对大鼠血小板进行预孵育,检测同位素标记的L-Arg在血小板转运的情况,以判断内源性硫化氢对血小板精氨酸转运的影响。结果不同浓度的H2S存在时均血小板对L-Arg转运速率均低于对照组;H2S可以快速降低血小板L-Arg转运速率;转运动力学显示H2S显著降低血小板L-Arg转运Vmax(P<0.05),但对Km值无明显影响。结论内源性硫化氢可能通过快速改变血小板L-精氨酸转运系统功能影响血小板功能。Objective To discuss the novel air neumrnodulator - H2 S＇ s effect on platelet function. Methods We use saturate H2S solution as donate of H2S. Rat＇s platelet were saturate with deferent density of H2S. The velocity of platelet L- Arg transport was measured by measuring radioactivity. Results At different concentrations H2S, the velocity of L-Arg transport was lower than that in controls（ P 〈 0. 01 ）. This effect has no time correlation. Conclusion Through a quick and efficient way, endogenous H2S can reduce platelet L-arg transport and effect on platelet derived NO produce.

关 键 词：内源性硫化氢 血小板 精氨酸转运 

分 类 号：R363.22[医药卫生—病理学]