银杏内酯对拟AD模型大鼠海马CA1区Aβ_(1-40)表达的影响  

A Sdudy of the Effect of Ginkgolides on Expression of Aβ_(1-40) in Hippocampal CA1 Region of Alzheimer's Disease-like Model Rats

作　　者：周灵贵[1] 曾昭毅[2] 黄江[1] 吕心瑞[2] 潘娅[2] 蒋乃昌[2] 

机构地区：[1]贵阳医学院多媒体形态学实验室,贵州贵阳550004 [2]贵阳医学院生理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2006年第4期313-316,共4页Journal of Guiyang Medical College

基　　金：贵州省优秀科技教育人才省长专业基金资助项目(S2001-3)

摘　　要：目的:研究银杏内酯对拟阿尔茨海默病(AD)模型大鼠海马CA1区Aβ1-40表达的影响。方法:采用冈田酸(OA)海马CA1区微量注射建立拟AD大鼠模型,3周后银杏内酯腹腔注射,水迷宫行为学试验检测学习记忆能力,免疫组织化学方法观察海马CA1区Aβ1-40的表达。结果:与拟AD模型组比较,银杏内酯组平均逃避潜伏期明显缩短(P<0.01),银杏内酯组海马CA1区Aβ1-40表达明显减少或消失(P<0.01)。结论:银杏内酯显著改善拟AD模型大鼠学习记忆能力,其机理可能是抑制海马Aβ1-40的表达及tau蛋白磷酸化,减轻OA对大鼠海马神经元的病理损害。Objective： To investigate the effects of ginkgolides on the expression of Aβ1-40 in hippocampal CA1 region of Alzheimer＇ s disease-like model rats. Methods： The experimental Alzheimer＇ s disease-like animal model was established by injiecting okadaic acid （OA） into the hippocampal CA1 region of rats. Then, ginkgolides were injected into the abdominal cavity of some of the rats （group GIN） every day for three weeks, while the other rats did not receive ginkgolides. The capabilities of learning and memory of the experimental animals were assessed through Morris Water Maze Behavior Test. The sinile plaques （SP）, the neurofibrillary tangles （NFT） of hippocampus were observed by Bielschowsky stain, and the expression of Aβ1-40 in hippocampal CA1 region was determined by immunohistochemistry methods. Results： Compared with control group, the average escape latency of experimental rats （group GIN） in place navigation test were shorter （P 〈 0. 01 ） and the expression of Aβ1 -40 in hippocampal CA1 region was significantly lower （ P 〈 0. 01 ）. Conclusions： Ginkgolides could alleviate the damage on the hippocampus neurons caused by OA through a possible mechanism of reducing the formation of Aβ1 -40 and the phosphorylation of Tau protain, and inhibiting the formation of SP and NFT.

关 键 词：阿尔茨海默病 二裂银杏 淀粉样Β蛋白 海马 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]