直肠癌患者生长激素受体表达水平与放疗敏感性的关系  

Correlation of Growth Hormone Receptor Expression to Preoperative Radiosensitivity of Rectal Cancer Patients

作　　者：吴晓宇[1] 许哲[1] 陈彻[1] 刘福坤[1] 黎介寿[1] 

机构地区：[1]南京大学医学院南京军区南京总医院全军普通外科研究所,江苏南京210002

出　　处：《癌症》2006年第9期1162-1167,共6页Chinese Journal of Cancer

摘　　要：背景与目的:生长激素(growthhormone,GH)能通过与生长激素受体结合激活一系列与DNA修复相关的信号通路从而对细胞产生放疗保护作用。本研究旨在探讨直肠癌患者生长激素受体(growthhormonereceptor,GHR)表达水平与放疗敏感性的关系。方法:应用免疫组化(immunohistochemistry,IHC)和逆转录聚合酶链反应(reversetranscription-polymerasechainraction,RT-PCR)方法检测98例直肠癌患者放疗前活检标本及相应的术后标本中GHR蛋白和mRNA的表达;放疗敏感性依据直肠癌减退分级标准评分。结果:免疫组化结果表明,放疗前直肠癌活检标本中GHR高表达与患者放疗耐受有显著相关性(rs=0.399,P<0.001);RT-PCR结果同样显示GHRmRNA阴性组具有更高的放疗敏感性(rs=0.398,P<0.001);而与放疗前活检标本相比,配对的放疗后标本中残留的癌细胞GHR表达水平显著升高(P<0.001)。结论:直肠癌患者放疗前活检标本中GHR高表达提示术前放疗敏感性低。BACKGROUND ＆ OBJECTIVE. Growth hormone （GH） could activate pathways involved in DNA repair process by binding to growth hormone receptor （GHR）, which is thought as one of the mechanisms of radioprotection. This study was to evaluate the correlation of GHR expression to radiosensitivity of rectal cancer. METHODS. The expression of GHR in biopsy specimens from 98 rectal cancer patients before preoperative irradiation and postoperative specimens was detected by immunohistochemistry （IHC） and reverse transcription-polymerase chain reaction （RT-PCR）. Radiosensitivity was evaluated according to Rectal Cancer Regression Grading. RESULTS. IHC revealed that GHR overexpression was positively correlated to poor response to radiotherapy （P〈0.001, rs=0.399）; RT-PCR also showed that radiosensitivity was significantly higher in GHR mRNA-negative group than in GHR mRNA -positive group （P〈0.001, rs=0.398）. Compared with the biopsy specimens before preoperative irradiation, the paired postoperative specimens showed significantly increased GHR expression in residual cancer cells （P〈0.001）. CONCLUSION： Detection of GHR expression before preoperative irradiation may predict radiosensitivity of rectal cancer.

关 键 词：直肠肿瘤 生长激素受体 术前放疗 放疗敏感性 免疫组化 逆转录聚合酶链反应 

分 类 号：R735.37[医药卫生—肿瘤]