成功老龄外周血单核细胞端粒酶活性的研究  

A study on telomerase activity of peripheral blood mononuclear cells of successful aging

作　　者：李晨虎[1] 李春波[2] 吴文源[2] 钱伊萍[3] 江三多[3] 张明园[3] 

机构地区：[1]上海市徐汇区精神卫生中心,200232 [2]同济大学附属同济医院 [3]上海市精神卫生中心

出　　处：《上海精神医学》2006年第4期199-201,共3页Shanghai Archives of Psychiatry

基　　金：国家自然科学基金(30170327);上海联合利华研究与发展基金项目(145)

摘　　要：目的探索成功老龄者外周血单核细胞端粒酶活性的特点。方法(1)共调查社区中70岁以上老人248名:成功老龄组121名,常态老龄组68名,MC I组59名。(2)应用“上海市社区老人健康问卷(S.A.2002)”,包括人口学资料、MMSE及ADL、躯体疾病指数等。(3)常规制备外周血单核细胞,运用TRAP法测定端粒酶活性。结果成功老龄组的端粒酶活性高于常态老龄、MC I组(2χ=12.50,P=0.00)。按照年龄进行分层后,成功老龄端粒酶活性亦显著性高于常态老龄及MC I(P<0.05)。结论成功老龄的外周血单核细胞具有较高的端粒酶活性。Objective： To explore telomerase activity of peripheral blood mononuclear cells（PBMC） of successful aging（SA）. Methods： （1）248 elderly people who lived in community and aged 70 years or over were investigated. There were 121 elder persons in successful aging group （SA） , 68 in usual aging group （UA）, 59 in mild cognitive impairment（MCI） group. （2）The instruments used were the Shanghai Health Survey for Elderly （Version S. A. 2002 ）, which included demographic details , Mini - mental status examination（ MMSE ）, Activity of Daily Living Scale （ADL）, physical disease list, etc. （3）The common methods for abstracting PBMC were used in all subjects and telomerase activity level was semiquantitatively assessed by telomeric repeat amplification protocol （ TRAP ）. Results： Telomerase activity was significantly higher in SA group than in UA and MCI groups（χ^2 = 12.50 ,P =0.00） , and after age layer the same result was got. Conclusion ： Telomerase activity of peripheral blood mononuclear cells was significantly higher in successful aging.

关 键 词：成功老龄 端粒酶 

分 类 号：R749.1[医药卫生—神经病学与精神病学]