全反式维甲酸对前列腺癌细胞生长及其连接蛋白43表达的影响  

Effects of all-trans retinoic acid on growth and connexin43 in prostate cancer cell

作　　者：陈卫国[1] 严春寅[1] 侯建全[1] 王恒兵[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,215006

出　　处：《江苏医药》2006年第9期836-837,共2页Jiangsu Medical Journal

基　　金：江苏省科技发展计划基金资助(BS2003013)

摘　　要：目的探讨全反式维甲酸（ATRA）对前列腺癌细胞生长增殖的抑制作用及其与连接蛋白（Cx）43之间的关系。方法不同浓度的ATRA处理体外培养的雄激素非依赖性人前列腺癌细胞株PC3后，分别应用光镜、甲基偶氮唑蓝（MTT）法和集落形成试验分析细胞生长和增殖的抑制情况；蛋白质免疫印迹法（Western blot）检测PC3细胞ATRA作用前后Cx43的表达。结果ATRA浓度为（10^-6～10^-4）mol／L时均可有效抑制PC3细胞的增殖（P〈0．05），光镜下可见细胞凋亡的特征性改变，抑制呈时间-剂量依赖性。ATRA处理前后PC3的Cx43表达量从无到阳性光带出现，且显色带随ATRA浓度增高而增强。结论ATRA能够抑制前列腺癌细胞的生长和增殖，其机制可能通过上调Cx43的表达来实现的。Objective To investigate the effects of all-trans retinoic acid（ARTA） on the growth of human prostate cancer cell and the expression of connexin 43（Cx43）. Methods The androgenunresponsive human prostate cancer cell PC3 cultured in vitro was treated with ARTA in different concentrations. The morphology of the cells was observed by microscopy. The cell growth and proliferation were measured by MTT assay. The expression of Cx43 in cancer cells was measured with Western blot. Results ARTA of 10^-6-10^-4 mol/L inhibited PC3 proliferation significantly in a dosedependent and time-dependent manner（P〈0.05）, and the morphologic characteristics of apoptotic cells were observed. The expression of Cx43 could not be detected in untreated PC3 with Western blot,but was detected in PC3 treated by ATRA, and upregulated obviously as the concentration of ATRA increased. Conclusion ARTA could significantly inhibit the growth of androgen unresponsive human prostate concer cell PC3, and this anti-tumor effect might be related to the up-regulation of Cx43 protein.

关 键 词：全反式维甲酸 前列腺癌 连接蛋白43 

分 类 号：R737.25[医药卫生—肿瘤]