白介素12重组腺病毒载体的构建及其抗肿瘤作用初步观察  

Construction of the mIL-12 Producing Recombinant Adenovirus Vector and Its Anti-tumor Effect

作　　者：鞠全荣[1] 黄丽华[1] 张孝卫[1] 杜德田[1] 耿秀兰[1] 刘宗印[1] 马力[1] 张如胜[1] 任东俊[1] 

机构地区：[1]济南市医学科学研究所,山东济南250013

出　　处：《中国肿瘤》2006年第9期614-616,共3页China Cancer

基　　金：济南市科技局立项课题:(021042)

摘　　要：[目的]构建携带小鼠白细胞介素12腺病毒载体(AdCMVmIL-12),并观察其对H22肿瘤的抑瘤效果。[方法]构建重组质粒pdlE1SP1BmIL-12,并用该质粒与质粒pJM17共转染293细胞后包装而成AdCMVmIL-12,并进行酶切法鉴定。同时制备肿瘤动物,通过肿瘤内局部注射AdCMVmIL-12,观察其抗肿瘤作用。[结果]酶切鉴定显示构建正确,其病毒滴度为1.3×109pfu/ml;当病毒滴度为100MOI时,mIL-12浓度达到3417ng/1×106细胞/24h。动物实验表明,注射AdCMVmIL-12的小鼠,肿瘤体积明显小于对照组(P<0.01),生存期显著延长。[结论]构建AdCMVmIL-12成功,肿瘤内局部注射可发挥抗肿瘤作用。[Purpose] To construct the mIL-12 producing recombinant adenovirus vector and observe its anti-tumor effect on H22 tumor. [Methods ] pdlEISP1 BmIL-12/p40/p35 was constructed. Then AdCMVmIL-1 2 was generated by contransfection of 293 cells with pdlEISP1BmIL-12/p40/ p35 and the adnenoviral packaging plasmid pJM17. The construction of AdCMVmIL-12 was examined by cleavage with restrictive enzymes, and the subcutaneous tumors of animal were developed and treated by direct intra-tumor injection of AdCMVmIL-12. [Results] The construction of AdCMVmIL-12 was right with the titers of 1.3×10^9pfu/ml. The mIL-12 expression was 3417ng/l × 10^6cell/24h when the titer was 100 MOI. The experiment showed that the sizes of the tumors in experiment group were much smaller than that in control group. [Conclusion] The mIL-12 recombinant adenovirus vector （AdCMVmIL-12）was constructed successfully and anti-tumor effect was achieved by direct intra-tumor injection.

关 键 词：白细胞介素12 重组腺病毒 基因表达 肿瘤 小鼠 

分 类 号：R73-36[医药卫生—肿瘤]