应用实时定量PCR检测儿童急性髓系白血病AML1-ETO融合基因  

Using real-time quantitative polymerase chain reaction detecting AML1-ETO fusion transcripts in children acute myeloid leukema

作　　者：金眉[1] 李志刚[1] 吴敏媛[1] 朱平[2] 张永红[1] 石惠文[1] 谢静[1] 赵玮[1] 高超[1] 

机构地区：[1]首都医科大学附属北京儿童医院血液病中心,100045 [2]北京大学第一医院血液科

出　　处：《中国小儿血液与肿瘤杂志》2006年第4期193-197,共5页Journal of China Pediatric Blood and Cancer

摘　　要：目的应用实时定量聚合酶链式反应方法,分析儿童急性髓系白血病中AML1ETO融合基因的表达水平,探讨AML1ETO表达水平的变化规律。方法采用实时定量PCR方法,以AML1ETO融合基因为靶分子,定量检测了28例急性髓系白血病患儿初诊时融合基因的表达量,分析了表达量与初诊临床特征的相关性,并对17例患者进行微小残留病定量分析。结果患者AML1ETO融合基因的表达范围在未治疗前达到17094～187800拷贝/106GAPDH拷贝;长期缓解的患者表达量降到0～46拷贝/106GAPDH拷贝;复发患者融合基因表达范围为62633.8～246000拷贝/106GAPDH拷贝。初诊时患者AML1ETO融合基因的表达量与临床特征无相关性(P均>0.05)。结论应用实时定量PCR技术对AML1ETO的定量检测是判断和追踪该类白血病疗效的有力指标,可以较好地反映患者的微小残留病的动态变化。Objective Using real-time quantitative PCR for the quantitative measurement of AML1- ETO fusion gene expression level in children with AML to explore the role of detecting AML1-ETO fusion gene expression level. Methods Quantification of AML 1-ETO fusion gene expression with real-time quantitative PCR was performed in 28 children with de novo AML. The relationship between expression level of AML 1-ETO fusion gene and clinical characteristics at presentation was analyzed. Minimal residual disease in 17 follow-up patients was quantitatively monitored for 10 to 218 weeks. Results At diagnosis, fusion gene expression levels ranged between 17094 and 187800 copies per 106 GAPDHH copies. Expression levels in continuous complete remission patients showed a decrease to 0 -46 copies per 106 GAPDH copies. At relapse, the fusion gene expression levels ranged between 62633.8 and 246000 copies per 106 GAPDH copies. No impact of clinical factors on AML1-ETO fusion transcripts at diagnosi was found （P 〉 0.05）. Conclusion Real-time quantitative PCR was a powerful tool for disease estimating and MRD monitoring in AML 1-ETO-positive AML.

关 键 词：实时定量PCR AML1-ETO 急性髓系白血病 儿童 微小残留病 

分 类 号：R733.71[医药卫生—肿瘤]