小麦材料PI31抗条锈性鉴定及其抗性基因SSR标记  

Identification and SSR mapping of a stripe rust resistance gene in wheat line PI31

作　　者：蒲宗君[1] 颜泽洪[1] 魏育明[1] 杨武云[2] 郑有良[1] 张增艳[3] 

机构地区：[1]四川农业大学小麦研究所,都江堰611830 [2]四川省农业科学院作物研究所,成都610066 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《植物病理学报》2006年第4期342-346,共5页Acta Phytopathologica Sinica

基　　金：四川省"十五"育种攻关项目;国家"八六三"计划项目(2003AA207099);四川省应用基础研究项目(05JY029-002-6);四川省科技厅招标项目(2005-09)

摘　　要：小麦材料PI31对我国当前流行的条锈菌小种条中30、31和32免疫;遗传分析表明,PI31携带一个显性抗条锈病基因。等位性测定显示,PI31所携带的抗条锈病基因与已知抗锈基因Yr5、Yr10和Yr15不等位。抗源系谱分析表明,该基因来源于叙利亚普通小麦品系叙18;故将此材料携带的抗条锈病基因暂定名为Yr-XU。利用分组分析(BSA)法,筛选到1个位于1BS的SSR标记WM S11-193 bp片段与Yr-XU紧密连锁,将Yr-XU定位于小麦1BS上;对F2分离群体142个单株分析结果表明,Yr-XU与WM S11-193 bp的遗传距离为2.1 cM,可将此标记用于小麦抗条锈病分子标记辅助育种。A wheat line, PI31, carried a novel single dominant candidate gene conferring high resistance to stripe rust isolates CYR30 ,CYR31 and CYR32. By using allelic test and pedigree analysis, it was found that the resistance gene in PI31 derived from Syria common wheat line Xul8 and was different from YrS, Yr10 and YrlS. This novel candidate gene was designated as Yr-XU. A SSR maker, WMS11-193 bp, on the 1BS was associated with Yr-XU. The genetic distance between Yr-XU and WMSll-193 bp was about 2.1 cM. Therefore, WMSll could be used as molecular marker assisted selection for Yr-XU and for pyramiding with other stripe rust resistance genes in wheat breeding program.

关 键 词：小麦条锈病 鉴定 等位测试 SSR标记 辅助选择 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]