龙葵总碱对肿瘤细胞膜钠泵及钙泵活性影响的研究  

The effect Effect of Total Alkaloids from Solanum nigrum on activities Activities of Na^+,K^+-ATPase and Ca^(++),Mg^(++)-ATPase in tumor Tumor cell Cell membranceMembrance

作　　者：季宇彬[1] 高世勇[1] 王宏亮[2] 邹翔[2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨150076

出　　处：《世界科学技术-中医药现代化》2006年第4期40-43,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金项目(30400591):中药龙葵中生物碱促进转染阴离子通道(AEI)膜段域K562细胞凋亡作用及机制研究;黑龙江省自然科学基金资助项目(D2004-13):龙葵总碱促进转染带3蛋白跨膜域K562细胞凋亡作用机制的研究

摘　　要：目的:观察龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na+,K+-ATPase及Ca++,Mg++- ATPase活性的影响。方法:荷瘤小鼠分为治疗组(高剂量、中剂量、低剂量)和对照组(生理盐水组、环磷酰胺组),分别测定各组肿瘤细胞膜的Na+,K+-ATPase及Ca++,Mg++-ATPase活性。结果:龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na+,K+-ATPase及Ca++,Mg++-ATPase活性均有明显的抑制作用,并且其抑制作用呈量效正相关。结论:龙葵总碱这一作用可能是其抗肿瘤作用机理之一。Objective：To observe the effect of total alkaloids （TA） from Solanum nigrum on the activities of Na^＋,K^＋-ATPase and Ca^＋＋,Mg^＋＋-ATPase in tumor cells＇membrance of S180 and H22 mice. Methods：Mice were divided into treated group （high, medium and low dosage） and control group （normal saline and cyclophosphamide）, and the activities of Na^＋,K^＋-ATPase and Ca^＋＋,Mg^＋＋ -ATPase in tumor cells＇membrance were examined measured respectively. Resuhs：TA showed pronounced inhibition on the activities of Na^＋,K^＋-ATPase and Ca^＋＋,Mg^＋＋-ATPase at in tumor cells＇membrance, and it showedand the inhibition showed dose-effect positive relationship. Conclusion ：This effect may be one of the mechanisms of its antitumor effect.

关 键 词：龙葵总碱 肿瘤细胞膜 钠泵 钙泵 

分 类 号：R730.2[医药卫生—肿瘤]