组织芯片技术在乳腺癌相关指标检测中的应用  

The Application of Microarray Immunohistochemical Technique in the Detection of the Index Correlated with Breast Cancer

作　　者：李洪涛[1] 宁连胜[2] 张斌[2] 

机构地区：[1]河北北方学院附属第一医院肿瘤外科,河北张家口075000 [2]天津医科大学附属肿瘤医院乳腺一科,天津300070

出　　处：《河北北方学院学报（医学版）》2006年第4期12-14,共3页Journal of Hebei North University:Medical Edition

摘　　要：目的:探讨组织芯片免疫组化方法在乳腺癌相关指标检测中的应用。方法:采用组织芯片免疫组化和传统免疫组化的方法分别检测了183例和30例乳腺癌组织中MMP-2,MMP-9,MMP-13的表达水平。结果:组织芯片免疫组化染色与传统免疫组化染色不存在统计学差异(P>0.05)。结论:高通量组织芯片免疫组化的方法在乳腺癌相关指标检测中可一次对多个样本进行多指标检测,大大节省了人力、物力和财力,值得进一步推广使用。Objective： To investigate the application of Microarray immunohistochemical technique in the detection of the index correlated with breast cancer. Methods： MMP-2, MMP-9 and MMP-13 were detected by Microarray immunohistochemical technique and traditional immunohistochemical technique in 183 patients and 30 patients with breast cancer respectively. Results： Microarray immunohistochemical technique has no significant difference compared with traditional immunohistochemical technique. （P〉0.05）. Conclusion： High-flux Microarray immunohistochemical technique can detect several indexes for many samples one time in the detection of the index correlated with breast cancer. It could save manpower, materials and financial resources. It is worth to be spread and applicated.

关 键 词：组织芯片 免疫组化 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]