血浆同型半胱氨酸水平及MTHFR基因多态与血管性痴呆的关系  

Relationship between vascular dementia,polymorphisms of MTHFR gene and plasma homocysteine levels

作　　者：毕胜[1] 潘尚哈[1] 隋丹[2] 王真奎[2] 王雪婷[2] 韩丽丽[2] 王振峰[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院中心实验室,黑龙江哈尔滨150001 [2]哈尔滨医科大学第一临床医学院神经内科,黑龙江哈尔滨150001

出　　处：《中风与神经疾病杂志》2006年第3期297-299,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨血浆同型半胱氨酸(H cy)水平及M THFR基因多态与血管性痴呆(VD)的关系。方法应用高效液相色谱仪和电化学检测法测定37例VD患者的血浆总H cy水平,并与40例同龄对照组及40例非痴呆脑梗死组比较,运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP)检测M THFR基因多态性,同时测定血浆叶酸及维生素B12水平。结果VD患者血浆总H cy水平显著高于同龄对照组(P<0.001)和非痴呆脑梗死组(P<0.05);M THFR基因型有3种,即纯合子(T/T)型,杂合子(T/C)型,纯合子(C/C)型。3组基因型和等位基因频率相比,差异均无显著性(P>0.05);VD组血浆叶酸及维生素B12水平明显低于同龄对照组(P<0.05)和非痴呆脑梗死组(P<0.05)。结论高同型半胱氨酸血症是VD发病的一个新的危险因素。Objective To study the relationship between plasma homocysteine （Hcy） levels, the polymorphisms of MTHFR gene and vascular dementia（VD）. Methods 37 VD patients,40 normal aged subjects and 40 patients with nondemented cerebral infarction were recruited in the study. Their plasma total Hcy levels were measured using high-performance liquid chromatography with electrochemical detection. The polymorphisms of MTHFR gene were analyzed using PCR-RFLP. Plasma folate and serum VitaminB12were determined for all of them. Results Mean total plasma Hcy concentrations were significantly higher in VD patients than in the normal elderly subjects （P〈0. 001）and patients with nondemented cerebral infarction. There were three kinds of genotype ：T/T （homozygous mutation）,T/C （heterozygous mutation）and C/C （wild-type）. There were no significance between the frequencies of the genotypes and alleles of MTHFR gene in three groups（P〉0.05）. Plasma folate and serum VitaminB12concentrations were significantly lower in AD group than in control group（P〈0.05）and patients with nondemented cerebral infarction. Conclusion Hyperhomocysteinemia may be a new risk factor for VD.

关 键 词：血管性痴呆 基因 半胱氨酸 叶酸 维生素B12 

分 类 号：R749.1[医药卫生—神经病学与精神病学]