多发性硬化与白细胞介素-1关系初步研究  

The relationship between interleukin-1 gene polymorphism and the serum level of interleukin-1β and the multiple sclerosis patients in the Northeast of China

作　　者：韩丽丽[1] 王雪婷[1] 王振峰[1] 隋丹[1] 王真奎[1] 毕胜[2] 

机构地区：[1]哈尔滨医科大学附属第一临床医学院神经内科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一临床医学院中心实验室,黑龙江哈尔滨150001

出　　处：《中风与神经疾病杂志》2006年第3期344-346,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨白细胞介素-1(IL-1),尤其是白细胞介素-1β(IL-1β)和白细胞介素-1受体拮抗剂(IL-1RA)基因多态性及IL-1β血清含量与东北地区汉族多发性硬化(M S)的关系。方法多聚酶链反应(PCR)对54例M S患者的血清进行IL-1β和IL-1RA基因多态性检测;酶联免疫吸附实验检测血清中IL-1β含量。结果IL-1β和IL-1RA基因型分布及等位基因频率在M S和对照组间无显著性差异(P>0.05)。IL-1β血清含量M S组比对照组显著增高(P<0.05)。结论IL-1β和IL-1RA基因多态性与M S易感性可能无相关性。IL-1β可能在M S发病中起到促进作用,参与启动炎症过程和组织损伤。Objective To implore the relationship between interleukin-1,especially interleukin-1β（IL-1β） and interleukin-1 receptor antagonist （IL-1RA）gene polymorphism and the serum level of IL-1β and the multiple sclerosis（MS）patients in the Northeast of China. Methods DNA of white blood cells from 54 subjects with MS and 60 healthy controls was extracted. Polymorphisms in IL-1β and IL-1RA were analyzed with PCR in order to find the difference of the genotype distribution and the allele frequency between the MS patients and the healthy controls. Results The genotype（CC,CT,TT）distribution of IL-1β was 44.4%,22.2%,and 33.4% in patients with MS and 46.7%,23.3% ,and 30.0% in controls（P〉0.05） ;while for the IL-1RA（Ⅰ/Ⅰ,Ⅰ/Ⅱ,Ⅱ/Ⅱ,Ⅰ/Ⅳ） was 81.5%,7.4%, 5.6%,5.5% in MS patients and 80.0%,8.3%,5.0%,6.7% in controls（P〉0.05）. The allele frequency of IL-1β in patients with MS were C 61.1% ,T 38.9% and 60.0% ,40.0% in controls ;white for the IL- 1RA（Ⅰ,Ⅱ,Ⅳ） were 85.2%,9.3%,5.5% in MS patients and 83.3%,10.0%,6.7% in controls. （P〉0.05）. The serum production of IL-1β was 146.83pg/ml in MS patients and was 96.23pg/ml in controls（P%0.05）. Conclusion The IL-1β and IL-1RA polymorphisms were not significantly associated with the susceptibility of MS,while IL-1β maybe play an active role in the MS and be involved in the start of inflammation progression and tissue damage.

关 键 词：IL-1Β IL-1RA 多发性硬化 基因多态性 

分 类 号：R744.51[医药卫生—神经病学与精神病学]