人乳腺癌细胞化疗敏感性与相关基因表达的关系  

Chemotherapy sensitivity and associated gene expression in human breast cancer cells

作　　者：魏玲[1] 宋现让[1] 王兴武[1] 柳永蕾[1] 

机构地区：[1]山东省肿瘤医院基础研究中心,山东济南250117

出　　处：《基础医学与临床》2006年第8期851-855,共5页Basic and Clinical Medicine

摘　　要：目的探讨人乳腺癌细胞化疗敏感性与多药耐药相关基因和凋亡调控基因表达的关系。方法MTT法检测5株人乳腺癌细胞株Bcap37、MCF-7、T47D、MDA-MB-231和MDA-MB-435对阿霉素(ADM)、顺铂(DDP)、丝裂霉素(MMC)、5-氟尿嘧啶(5-Fu)、卡氮芥(BCNU)的敏感性。流式细胞术检测多药耐药基因P-糖蛋白(P-GP)、谷胱甘肽-S-转移酶-π(GST-π)、肺耐药蛋白(LRP)、多药耐药相关蛋白(MRP)和甲基鸟嘌呤甲基转移酶(MGMT)等耐药相关基因和凋亡调控基因FAS、BCL-2、P53和P16的表达。结果不同人乳腺癌细胞株对同一药物敏感性差异较大。相关分析表明,细胞株对5-Fu的敏感性与P16表达呈正相关(P<0.01),对其余药物的敏感性与所检基因的表达水平无关。结论人乳腺癌细胞株对5-Fu的敏感性与P16表达有关。Objective To explore the relationship of chemotherapy sensitivity and expression of multidrug resistance genes and apoptosis regulation genes in human breast cancer cell lines. Methods MTT assay was used to detect the sensitivity to adriamycin （ ADM ）, cisplatinum （ DDP）, mitomycin C （ MMC ）, fluorouracilum （ 5-Fu）, carmustine （BCNU） in five breast cancer cell lines including Bcap37, MCF-7, T47D, MDA-MB-231 and MDA-MB-435. Multidrug resistance genes including P-glycoprotein （P-GP）, Glutsthione-s-transferases-Л （GST-Л）, Lung resistance protein （LRP）, multidrug resistance related protein （MRP） , MGMT and apoptosis regulation genes FAS , BCL-2, P53 and P16 were examined by flow cytometry（FCM）. Results The chemotherapy sensitivity was obviously divergent in different breast cancer cell lines. The correlation analysis showed that there were positive correlations between the sensitivity to 5-Fu in breast cancer cell lines and the expression of P16. There was no correlation among the sensitivity to other drugs and expression of other genes. Conclusions The sensitivity to 5-Fu is related to the expression of P16 in breast cancer cell lines.

关 键 词：乳腺癌细胞株 流式细胞术 化疗敏感性 多药耐药相关基因 凋亡调控基因 

分 类 号：R737.9[医药卫生—肿瘤]