反相高效色谱法测定蜂王浆中10-HDA的含量  

Determination of 10-HDA in royal jelly by RP-HPLC

作　　者：罗小凤[1] 邹盛勤[2] 刘传安[3] 

机构地区：[1]宜春学院医学院,宜春336000 [2]宜春学院生物工程研究所,宜春336000 [3]宜春学院物理科学与工程学院,宜春336000

出　　处：《食品科技》2006年第8期249-251,共3页Food Science and Technology

摘　　要：建立反相高效液相色谱法测定了蜂王浆中10-HDA的含量,同时使用光电二极管阵列检测器分析10-HDA色谱峰的纯度。色谱柱为NucleodurC18柱(4.6mm×250mm,5μm),流动相为甲醇∶2%磷酸溶液(55∶45,v/v),流速1.0mL/min,检测波长212nm,柱温25℃。10-HDA在1.384￣13.84μg范围内线性关系良好(r=0.9999),10-HDA平均回收率为98.2%,RSD为1.0%。方法简便准确、重现性好、线性范围宽,可用于评价蜂王浆的质量。Determination method of 10-HDA in royal jelly by reversed-phase high performance liquid chromatograph was established and at the time the purity of 10-HDA peak was detected by means of Photodiode Array Detector（PAD）. The chromatographic column, Nucleodur C18 column （4.6mm×250mm, 5μm）, methanol-2% phosphoric acid solution （55：45, v/v） mobile phase with 1.0mL/min flow rate, the detected wavelength （212nm）, and the column temperature（25%） were adopted. The calibration curve of 10-HDA was linear under the content of 1.384-13.84μg, the correlation coefficient was over 0.9999. The average recovery of 10-HD was 98.2% and RSD was 1.0%. The method is simple, accurate, reproducible and has wider linear range. The method can be adapted to evaluate the quality of royal jelly.

关 键 词：蜂王浆 10-HDA 光电二极管阵列检测器 反相高效液相色谱 含量测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]