荧光实时定量RT-PCR在口腔鳞癌细胞角蛋白19表达研究中的应用  

Relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time RT-PCR

作　　者：钟来平[1] 胡济安[1] 赵士芳[1] 许则丰[2] 平飞云[3] 陈关福[3] 

机构地区：[1]浙江大学医学院附属口腔医院口腔颌面外科,杭州310031 [2]浙江大学肿瘤研究所 [3]浙江大学医学院附属第二医院口腔颌面外科

出　　处：《中华口腔医学杂志》2006年第9期553-555,共3页Chinese Journal of Stomatology

摘　　要：目的探讨荧光实时定量逆转录-聚合酶链反应(RT-PCR)在口腔鳞癌组织中细胞角蛋白19(CK19)相对表达量研究中的应用。方法用 CK19mRNA 的荧光定量 RT-PCR 检测31例口腔鳞癌患者的癌组织和癌旁组织,用2^(-△△Ct)公式计算癌组织相对癌旁组织 CK19mRNA 相对表达的倍数。结果口腔鳞癌癌组织 CK19mRNA 水平是癌旁组织的2.21倍,扩增片段特异,CK19mRNA 水平与病理分化程度相关,分化越差,水平越高。结论荧光实时定量 RT-PCR 在口腔鳞癌组织 CK19相对表达量研究中具有一定的临床价值。Objective To investigate the relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time reverse transcription-polymerase chain reaction（ （RT-PCR）. Methods CK19mRNA level was detected by fluorescent quantitive real-time RT-PCR in cancerous and para-eaneerous tissues from 31 oral squamous cell carcinoma patients. According to the 2^△△Ct equation, the relative quantification fold of CK19mRNA level was calculated in cancerous tissues compared with para-eaneerous tissues. Results CK19mRNA levels in cancerous tissues were 2.21 folds higher than those in para-eaneerous tissues, and the amplieon was specific. CK19mRNA level in cancerous tissue correlated significantly with pathological differentiation degree, the poorer the differentiation was, the higher the CK19mRNA level became. Conclusions Fluorescent quantitive real-time RT-PCR is accurate and reliable in the detection of relative quantification of CK19 transcription in oral squamous cell carcinoma tissues.

关 键 词：聚合酶链反应 癌 鳞状细胞 细胞角蛋白 

分 类 号：R739.8[医药卫生—肿瘤]