Scleraxis在牙周韧带细胞、牙龈成纤维细胞中的表达  

Expression of Scleraxis in human periodontal ligament cells and gingival fibroblasts

作　　者：刘琼[1] 谢昊[2] 李五一[2] 李成章[2] 

机构地区：[1]大连市口腔医院牙周科,辽宁116021 [2]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079

出　　处：《中华口腔医学杂志》2006年第9期556-558,共3页Chinese Journal of Stomatology

基　　金：湖北省自然科学基金资助项目(2004ABA178)

摘　　要：目的研究碱性螺旋-环-螺旋转录因子 Scleraxis 能否在人牙周韧带细胞、牙龈成纤维细胞、骨髓基质细胞中表达,及其与牙周韧带细胞分化能力的关系。方法体外培养人牙周韧带细胞、牙龈成纤维细胞和骨髓基质细胞,RT-PCR 法检测牙龈成纤维细胞、骨髓基质细胞及不同代次牙周韧带细胞中 Scleraxis 的表达。结果 Scleraxis 在牙周韧带细胞、骨髓基质细胞及牙龈成纤维细胞中的表达差异有统计学意义(P<0.05)。其 Scleraxis 与β-肌动蛋白吸光度比值分别为0.877±0.024,0.438±0.031,0.313±0.083。Scleraxis 在牙周韧带细胞中的表达最强,在牙龈成纤维细胞中表达最弱。牙周韧带细胞中 Scleraxis 的表达随培养代次的增加而减弱。结论 Scleraxis 可表达于体外培养的牙周韧带细胞、牙龈成纤维细胞和骨髓基质细胞,Scleraxis 可能在牙周韧带细胞的分化过程中起重要作用。Objective To study if Scleraxis, a basic helix-loop-helix type transcription factor, could be expressed in human periodontal ligament cells （hPDLC）, bone marrow cells （hBMSC） and gingival fibroblasts （hGF） , and to investigate if Scleraxis was involved in hPDLC differentiation. Methods hPDLC , hBMSC and hGF were cultured. Expression of Scleraxis in hPDLC from different passages and in hBMSC and hGF was analyzed by reverse transcription polymerase chain reaction （RT-PCR）. Results Scleraxis expression in hPDLC, hBMSC and hGF were significantly different （ P 〈 0.05 ）. The A values of Scleraxis/β-actin in these kinds of cells were 0. 877 ± 0. 024, 0. 438 ± 0. 031,0. 313 ± 0. 083 , respectively. The expression of Scleraxis was the highest in hPDLC and lowest in hGF. Scleraxis expression of hPDLC decreased with increase of passages in culture. Conclusions Scleraxis was expressed in hPDLC, hBMSC and hGF in vitro, and may play an important role in differentiation of hPDLC.

关 键 词：细胞分化 转录因子 牙周韧带细胞 

分 类 号：R78[医药卫生—口腔医学]