大豆异黄酮和补充钙对去卵巢大鼠骨密度及肝IGF-1 mRNA表达的影响  

Soy isoflavones with supplemental calcium provide protection against the loss of bone mass and influnece insulin-like growth factor (IGF)-Ⅰ after ovariectomy in rat

作　　者：程茅伟[1] 刘家发[1] 易国勤[1] 章锡平[2] 刘烈刚[2] 朱清华[2] 

机构地区：[1]湖北省疾病预防控制中心卫生检测检验防护所营养与食品安全部,武汉430079 [2]华中科技大学同济医学院营养与食品卫生学系

出　　处：《中华预防医学杂志》2006年第5期328-331,共4页Chinese Journal of Preventive Medicine

摘　　要：目的利用卵巢切除大鼠模型,研究大豆异黄酮和钙对卵巢切除大鼠骨密度及肝脏IGF-1基因表达的影响。方法将6月龄雌性 SD 大鼠,按体重随机分成5组:假手术组(Sham)、卵巢切除阴性对照组(OVX)、单纯大豆异黄酮组(SI)、单纯碳酸钙组(Ca)、大豆异黄酮加碳酸钙组(SI+Ca)。所有大鼠饲以钙含量为3.732g/kg 的低钙饮食喂养12周。实验结束时,利用双能 X 线骨密度扫描仪测量右侧股骨骨密度(BMD),采用逆转录.聚合酶链反应(RT-PCR)方法测定肝脏胰岛素样生长因子-1(IGF-1)基因表达水平。结果 SI+Ca 组股骨远心端 BMD 为(0.263±0.007)g/cm^2,Sham组为(0.267±0.008)g/cm^2,两组间差异无统计学意义,但这两组 BMD 均显著性高于 OVX(0.245±0.005)g/cm^2、SI(0.258±0.011)g/cm^2和 Ca(0.255±0.004)g/cm^2组(P<0.05)。肝脏 IGF-1mRNA 表达水平在 Sham(0.200±0.023)g/cm^2、SI(0.278±0.019)g/cm^2、Ca(0.302±0.026)g/cm^2及SI+Ca(0.231±O.025)g/cm^2组中均显著性低于 OVX(0.362±0.031)g/cm^2,P<0.05,SI+Ca 组IGF-1mRNA 表达水平(0.231±0.025)g/cm^2低于 SI(0.278±0.019)g/cm^2和 Ca(0.302±0.026)g/cm^2两组,并差异有统计学意义。结论 SI+Ca 能比单纯喂饲 SI或 Ca 更好地防止卵巢切除大鼠股骨 BMD 的减小。37.95mg/kg 剂量的 SI 能够显著抑制由于卵巢切除引起的肝脏 IGF-1 mRNA 表达水平的上升。Objective To compare the effects of soy isoflavone with supplemental calcium to soy isoflavone or Ca alone on preservation of bone mineral density （ BMD ） and the expression of insulin-like growth factor （IGF）-Ⅰ. Methods Sprague-Dawley （SD） female Rats,6 months old, were ovariectomized and randomized into five groups： sham-operated group（ n = 10 ） or ovx （n = 40 ） group. Shams were fed a 3. 272 g/kg Ca diet. Ovx rats were randomized to a 3. 272 g/kg Ca diet alone （OVX） or with soy isoflavone （SI） extract （37.95 mg/kg, bw） or to a supplemental Ca diet （ Ca, 4. 676 g/kg） alone or a supplemental Ca diet with the isoflavone extract （ SI ＋ Ca） for 12 weeks. BMD of femur was measured by scanner of bone mineral density. The level of IGF-1 mRNA expression was measured by reverse transcriptase-polymerase chain reaction（RT-PCR） , respectively. Results There was no significant difference between group Sham （ 0. 267 ± 0. 008 ） and group SI ＋ Ca （0. 263 ± 0. 007 ） g/cm^2 （ P 〉 0. 05 ） on femur BMD of distal end. Femur BMD of distal end in group Sham and group SI ＋ Ca was greater （ P 〈 0. 05 ） as compared to group OVX （0.245±0.005） g/cm^2, SI（0.258±0.011）g/cm^2 or Ca（0.255±0.004）g/cm^2,p 〈0.05. The liver tissue IGF-1 mRNA contents （IGF-1 cDNA/B-actin cDNA） were significantly decreased in group Sham （0. 200±0. 023） g/cm^2 ,SI（0. 278±0. 019） g/cm^2 ,Ca（0. 302±0. 026） g/cm^2 or SI ＋ Ca（0. 231±0. 025） g/cm^2 as compared to group OVX （0. 362 ± 0. 031 ） g/cm^2,P 〈 0. 05 ; The liver tissue IGF-1 mRNA contents （IGF-1 cDNA/B-actin cDNA） were significantly decreased in group SI ＋ Ca （0. 231 ± 0. 025 ） g/cm^2 compared to group SI （0. 278 ± 0. 019 ） g/cm^2 and Ca （ 0. 302 ± 0. 026 ） g/cm^2, P 〈 0. 05. Conclusion Soy isoflavones combined with supplemental Ca are more protective against the loss of femur BMD than soy isoflavones or supplemental Ca diet alone. The dose of SI（37.95 mg/kg, bw）

关 键 词：异黄酮类 钙 骨密度 胰岛素样生长因子-1 卵巢切除术 大鼠 

分 类 号：R151[医药卫生—营养与食品卫生学]