吸烟与非吸烟肺癌患者中P53基因突变的检测  

Detection of P53 gene mutation in lung carcinomas of smoking and non-smoking patients

作　　者：聂岚[1] 朱惠刚[1] 

机构地区：[1]上海医科大学环境卫生教研室

出　　处：《中国环境科学》1996年第4期311-314,共4页China Environmental Science

摘　　要：Ｐ５３基因定位于人类第１７号染色体的短臂，野生型Ｐ５３基因是肿瘤抑制基因。这一基因正成为研究人类肿瘤ＤＮＡ的主要目标。作者运用聚合酶链反应－单链构象多态性技术（ＰＣＲ－ＳＳＣＰ），研究了４５例石蜡包埋的肺癌组织标本中肿瘤抑制基因Ｐ５３基因突变，结果发现在Ｐ５３基因的第５～８外显子中共检测到１９例Ｐ５３基因突变的样本，检出率为４２．２％，并且发现吸烟的肺癌患者中Ｐ５３基因突变的检出频率明显高于非吸烟的肺癌患者。P53 gene is localized in the short arm of human chromosome 17. The wild type P53 gene is tumour suppressor gene. It is becoming the target to study human tumour DNA and the hot spot in studying of molecular tumourology. In this study, we analyse the mutation of P53 gene in 45 human lung tumour tissues embedded in paraffin by using methods of PCR (Polymerase Chain Reaction) and SSCP(Single-Strand Conformation Polymorphism). The results showed that P53 gene mutations were detected in 19 samples from exon5 to exon8 has the detective percentage is 42. 2%, and also the percentage of P53 gene mutation in patients of cigarette smoking is hinger than that in non-smoking.

关 键 词：吸烟 肺癌 肿瘤抑制基因 P53基因 检测 

分 类 号：R734.204[医药卫生—肿瘤] R163[医药卫生—临床医学]