益气活血方对肺癌细胞株上皮细胞粘附分子表达及侵袭能力的影响  

Effect of Yiqi Huoxue Formula on Expression of E-CD and Invasion of Human Lung Cancer Cell Lines in Vitro

作　　者：王炳胜[1] 石德光[1] 翟书悦[1] 刘登凯[1] 张秀丽[1] 刘秀芳[1] 张海[1] 

机构地区：[1]中国人民解放军第251医院肿瘤科,河北张家口075000

出　　处：《中国中医药信息杂志》2006年第9期22-24,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的观察益气活血中药对肺癌细胞株体外生长抑制,上皮细胞粘附分子(E-CD)表达及侵袭能力的影响。方法采用细胞计数、S-P免疫组织化学、图像分析及侵袭小室等方法,观察小细胞肺癌细胞株NC-H446姜益气活血中药、全反式维甲酸(ARTA)处理不同时间后细胞生长状况、E-CD表达及侵袭能力的影响。结果姜两干预因素下细胞生长抑制,形态改变。中药干预下,NC-H446细胞株E-CD阳性表达率由原来的12%±8%提高到34%±9%,积分光密度(IOD)由78.5±16.9增加到200.3±21.7,前后相比具有明显差异(P<0.01);穿过基质胶的细胞数分别为126.5±43.61,与对照组相比差异显著(P<0.01);与ARTA相比无明显差异。结论益气活血中药能明显抑制NC-H446细胞株的生长,能促进E-CD的正常表达,减弱肿瘤细胞的侵袭转移能力;中药姜上述作用方面可代替ARTA。Objective To observe the effects of Chinese herbal medicine of Yiqi Huoxue （YQHX） on proliferation and expression of E-CD and invasion of human lung cancer cell lines - small cell lung cancer （NC-H446） in vitro. Methocls By cell measured method, immunohistochemistry method, technique of image and Boyden Chamber method, the effects of YQHX or all trans-tretinoin acid （ARTA） on proliferation and expression of E-CD and invasion of NC-H446 cell lines with different time cultured by YQHX or ARTA in vitro were studied, Results The proliferation of NC-H446 were inhibited significantly by 5% YQHX or 10^-5 mmol/L ARTA （P〈0.01）, but there is not significantly differentiation between YQHX and ARTA （P〉0.05）. The shape and structure of cell NC-H446 was occurred variant. YQHX enhanced remarkably expression of E-CD of NC-H446 with 36 hour cultured （P〈0.01）. There was no significant difference between YQHX and ARTA （P〉0.05）. The quantity of NC-H446 cells crossed Matrigel and polyvinylpyrrolidone-free polycarbonate filter were more than the control group （P〈0.01）. There was significant difference between YQHX and ARTA （P〉0.05）. Conclusion YQHX can inhibit the proliferation, enhance expression of E-CD on NC-H446, and decrease invasion and metastasis of NC-H446.

关 键 词：钙粘蛋白 益气活血中药 肺癌细胞/NC-H446培养 

分 类 号：R743.3[医药卫生—神经病学与精神病学]