MnSOD 9Ala/Val基因多态性与冠心病的关系  

Association between manganese superoxide dismutase(MnSOD)9 Ala/Val genetic polymorphisms and coronary artery disease

作　　者：迟东升[1] 凌文华[1] 马静[1] 夏敏[1] 候孟君[1] 王庆[1] 朱惠莲[1] 唐志红[1] 余小平[1] 

机构地区：[1]中山大学公共卫生学院医学营养系,广州510080

出　　处：《中国公共卫生》2006年第9期1078-1080,共3页Chinese Journal of Public Health

基　　金：中国博士后科学基金项目(2004036155);广东省自然科学基金团队项目(015042)

摘　　要：目的探讨锰超氧化物歧化酶9 Ala/Val(MnSOD9 Ala/Val)基因多态性与冠心病、总超氧化物歧化酶(T-SOD)和锰超氧化物歧化酶(MnSOD)活性以及丙二醛(MDA)浓度的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测262例冠心病患者和100例对照组的MnSOD9 Ala/Val基因多态性的基因型,采用比色法测定血浆T-SOD、MnSOD活性以及MDA浓度。结果与对照组比较,冠心病患者的血浆T-SOD和MnSOD活性[(23.61±16.51)U/ml与(44.01±22.68)U/ml,P<0.001和(21.56±13.11)U/ml与(28.79±8.65)U/ml,P<0.001]明显降低,MDA浓度显著增高[(2.47±0.73),(2.15±0.55)nmol/ml,P<0.01];冠心病患者的MnSOD 9 AA基因型和A等位基因频率较对照组明显增多(64.2%与43.0%,P=0.001和80.0%与67.0%,P=0.014);MnSODAA基因型的MnSOD活牲较VV基因型明显降低[(22.87±13.47)与(32.04±9.19)U/ml,P<0.01)];MnSOD与MDA负相关(r=-0.15,P<0.01)。结论冠心病患者的血浆T-SOD和MnSOD活性明显降低,MDA浓度显著增高;MnSOD 9 Ala/Val基因多态性AA基因型的血浆MnSOD活性降低。Objective To study the associations between manganese superoxide dismutase 9 Ala-Val genetic polymorphisms（ MnSOD 9 Ala/Val）and coronary artery disease（CAD）, plasma activities of total superoxide dismutase（T-SOD）, MnSOD, as well as plasma concen tration of maleic dialdehyde（MDA）. Methods Using PCR-RFLP method to identify genotype of MnSOD 9 Ala/Val genetic polymorphisms, and using colorimetry to detect plasma activities of T-SOD, MnSOD and plasma concentration of MDA in 262 CAD patients and 100 controls. Results Compared with controls, the plasma activities of T- SOD and MnSOD reduced obviously[ （23.61 ± 16.51）U/ml vs（44.01 ± 22.68）U/ml and（21.56 ± 13.11） U/ml vs（28.79 ±8.65）U/ml, P〈 0.001 and P〈 0.001, respectively）, while plasma MDA concentration in creased markedly[（2.47± 0.73） nmol/ml vs（2.15 ± 0.55 ）nmol/ml, P 〈 0.01 ）. There were more AA genotype and A allele of MnSOD 9 Ala/Val genetic polymorphisms（64.2 % vs 43.0%, P〈 0.001 and 80.0% vs 67.0%, P = 0. 014, respectively）than controls in CAD patients. The activity of plasma MnSOD depressed in individuals with MnSOD AA genotype compared with those with VV genotype（22.87 ± 13.47 vs 32.04 ± 9.19 U/ml, P 〈 0.01 ）. MnSOD correlated negatively with MDA（ r = - 0.15, P 〈 0.01 ）. Conclusion The plasma activities of T-SOD and MnSOD reduce obviously, in contrary, plasma concentration of MDA increases markedly in CAD patients. The activity of plasma MnSOD depress in individuals with MnSOD AA genotype.

关 键 词：冠心病 锰超氧化物歧化酶 基因多态性 

分 类 号：R394[医药卫生—医学遗传学]