热休克蛋白Hsp70在抗原呈递过程中的作用  

Roles of heat shock protein 70 family in antigen presentation

作　　者：黄唯燕[1] 余权[2] 赵玫[2] 

机构地区：[1]武汉大学生命科学学院2003级生物技术基地班,武汉430072 [2]中国医学科学院肿瘤医院肿瘤研究所

出　　处：《中国公共卫生》2006年第9期1084-1085,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(3017108930570904)

摘　　要：目的研究热休克蛋白Hsp70家族在抗原呈递中的作用,探讨其与肿瘤免疫反应的关系。方法利用反义RNA技术,将反义Hsp70、反义Hsc70、B7表达质粒导入肿瘤细胞B16中,24 h后检测细胞中热休克蛋白表达水平。利用混合淋巴细胞培养,检测T淋巴细胞增殖能力。结果在黑色素瘤细胞B16中转染pLXSN-antihsp70、pLXSN-antihsc70及2者共转染,转染反义质粒能够抑制B16细胞中Hsp70/Hsc70的表达,导致Hsp70表达下调,其刺激T淋巴细胞增殖能力均大幅下降。而将pLXSNmB7转染入B16细胞中,其刺激T细胞增殖的能力均有所上升。转染pLXSNmB7后,B16细胞中Hsp70/Hsc70的表达量有所提高。结论抑制各热休克蛋白Hsp70家族的表达能够导致T细胞增值数降低。在肿瘤细胞中导入B7分子能够提高Hsp70的表达水平,从而提高其抗原呈递的能力。Objective To study the ability of Heat shock protein 70 family in the procession of the antigen presentation and discuss the relationship of Hsp70 family and the tumor immunogenesis, Methods Antisense hsp70, antisense hsc70 and B7 express plasmid were transfected into tumor cell line B16, and the expression of heat shock protein 70 and the effect of inhibiting Hsp on T lymphocyte proliferation were detected after 24 hours, Results The expression of heat shock protein 70 were inhibited in B16 after transfection of pLXSN- antihsp70, pLXSN- antihsc70, the efficiency of T cell proliferation was declined. In the B16 cell with pLXSNmB7, hsp70 expression were promoted and the efficiency of T cell proliferation was increased. Conclusion Inhibiting the expression of Hsp70 family can induce the decline of the efficiency of T cell proliferation. Transfecting pLXSNmB7 into tumor cell can improve the expression of Hsp70 family and the ability of antigen presentation,

关 键 词：热休克蛋白70(Hsp70) 抗原呈递 肿瘤免疫反应 

分 类 号：R73[医药卫生—肿瘤]