热休克蛋白90在抑制细胞凋亡中的作用  

Effect of heat shock protein 90 on apoptosis synergistically induced by tumor necrosis factor-α and cycloheximide in NIH3T3 cells

作　　者：戴顺东[1] 张秀伟[1] 范垂峰[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2006年第3期233-234,共2页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30470764)

摘　　要：目的探讨热休克蛋白90(HSP90)是否能够抑制肿瘤坏死因子α(TNF-α)和放线菌酮(CHX)协同诱导的细胞凋亡。方法采用电穿孔技术建立稳定过表达HSP90的细胞克隆,应用激光共聚焦显微镜和流式细胞仪观察TNF-α/CHX协同诱导的细胞凋亡。结果在稳定过表达HSP90的小鼠成纤维细胞系NIH3T3中,HSP90能够抑制TNF-α/CHX协同诱导的细胞凋亡;在TNF-α/CHX协同诱导的细胞凋亡中,HSP90持续稳定存在12h以上。结论HSP90能够抑制TNF-α/CHX协同诱导的细胞凋亡。Objective： To investigate whether heat shock protein （HSP90） could suppress the apoptosis synergistically induced by tumor necrosis factor-α （TNF-α） and cycloheximide （CHX）. Methods： The cell clone with stable HSP90 overexpression was estabfished. The TNF-α/CHX induced apoptosis was detected by using laser confocal microscope and flow cytometry. Results： HSP90 suppressed the TNF-α/CHX induced apoptosis in NIH3T3 cells with stable HSP90 overexpression and existed stably for more than 12 hours. Conclusion： HSP90 could suppress TNF-α/CHX induced apoptosis.

关 键 词：热休克蛋白90 肿瘤坏死因子Α 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]