吲哚美辛与雄激素对喉癌细胞Hep-2增生与凋亡的影响  

Preliminary study on effect of indomethacin and mibolerone on Hep-2 cell proliferation and apoptosis

作　　者：王为方[1] 孙吉凤[1] 赵晓东[2] 刘剑凯[1] 赵学俭[3] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学教研室,长春130021 [2]吉林大学中日联谊医院耳鼻咽喉头颈外科 [3]吉林大学基础医学院病理生理教研室,长春130021

出　　处：《肿瘤研究与临床》2006年第9期585-587,590,共4页Cancer Research and Clinic

基　　金：中国博士后科学基金资助项目(2004035566)

摘　　要：目的探索吲哚美辛(IND)与雄激素(Mib)对喉癌细胞增生与凋亡的作用。方法Hep-2细胞暴露于含IN(D125,250,500μmo/lL),或Mi(b1,10,100nmo/lL),或IND与维持剂量的Mib(1nmo/lL)同时存在的培养基中,分别培养24h;用MTT法、锥虫蓝染色法与流式细胞仪分析法分别检测细胞增生、细胞活力、细胞周期和细胞凋亡。结果IND使Hep-2细胞增生及细胞活力明显降低,S期细胞含量明显增加,且出现凋亡峰;Mi(b100nmo/lL)明显促进Hep-2细胞增生;Mi(b1nmo/lL)与IN(D250μmo/lL)同时作用,下调细胞增生的抑制率,明显减少死细胞数,但S期细胞含量以及细胞凋亡比率明显上升。结论IND强烈抑制Hep-2细胞增生与细胞活力,阻滞细胞从S期向G/2M期转化,并诱导其凋亡;维持剂量的Mib具有保护细胞膜的作用,而另一方面又具有促进IND诱导细胞凋亡的作用。Objective To study effects of indomethacin and androgen hormone on proliferation and apoptosis of laryngeal cancer cell Hep-2. Methods Hep-2 cells were exposed to indomethacin at concentrations of 125, 250, 500 μmol/L, or mibolerone at concentrations of 1, 10, 100 nmol/L, or both indoroethacin and mibolerone at maintenance concentration of 1 nmol/L, and cultured for 24 h. MTT, trypan blue exclusion experiments and propadiene iodide staining were used to determine cell proliferation, cell viability and cell cycle distribution as well as apoptosis, respectively. Results The cell proliferation and viability were down regulated by indomethacin and the cells in S phase were increased and appeared obvious apoptosis peaks; mibolerone in 100 nmol/L increased the cell proliferation; mibolerone in 1 nmol/L combined with indoroethacin in 250 μmol/L decreased the inhibition rate of proliferation, while the cell viability increased and the cells in S phase and the cells with apoptosis were increased significantly. Conclusion Indomethaein strongly inhibits the cell proliferation and viability, arrests cells in S phase and induces cell apoptosis; mibolerone in 1 nmol/L protects the cell membrane, on the other hand promotes apoptosis induced by indomethacin.

关 键 词：吲哚美辛 雄激素 喉癌细胞HEP-2 增生 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]