再生水灌溉对草坪土壤微生物群落的影响  

Influence of reclaimed water irrigation on microbial community on lawn soil

作　　者：郭逍宇[1] 董志[2] 宫辉力[1] 

机构地区：[1]首都师范大学资源环境与旅游学院资源环境与地理信息系统北京市重点实验室,北京100037 [2]首都师范大学生命科学学院,北京100037

出　　处：《中国环境科学》2006年第4期482-485,共4页China Environmental Science

基　　金：北京市教育委员会资助项目(KE200410028014)

摘　　要：为探讨再生水替代自来水进行草坪灌溉的可行性,采集了北京市市政再生水草坪灌区及自来水对照区根际、根系层和非根系层土壤样品,通过可培养微生物在不同培养基上数量的变化,研究再生水对草坪根部不同空间微生物种群密度的影响;对根际层LB培养基10-4稀释度平板上分离的细菌单菌落提取基因组DNA,扩增16SrDNA片段并用限制性内切酶HinfⅠ对PCR产物进行扩增rDNA限制性分析(ARDRA),并采用综合多样性指数(H′),丰富度指数(R2)和均匀性指数(E1)等指标评价再生水灌溉对根际微生物多样性的影响.结果表明,与对照相比,再生水草坪灌溉有利于草坪草根际微生物数量的增加,尤其是有利于芽孢杆菌的增加,但对根系及非根系层微生物无显著影响.再生水灌溉使得微生物群落结构及多样性发生相应变化.这种变化体现为优势类群及亚优势类群多度增加,对再生水敏感的非优势类群失去原有的地位,另外一部分偶见类群出现或消失,群落多样性最终增加.For exploring the feasibility of using the reclaimed water to replace the tap water for lawn irrigation, the soil samples of the root edge, layer and non-layer were collected in the Beijing municipal reclaimed water lawn irrigation area and tap water contrast area; and through the changes of the amount of microbe capable of culture in different culture basis, the influence of the reclaimed water on the microbe population density on the lawn root different spaces was studied. The amplified rDNA restriction analysis（ARDRA） was carried out on the draw of gene group DNA of single bacteria community separated from that of the root edge layer LB culture basis of 10^-4 dilution, amplified 16S rDNA section and using restrictive Hinf I on PCR product; and adopting synthetical index number of diversity（H′）, index number of richness（R2） and index number of evenness（E1） to evaluate the influence of reclaimed water irrigation on the variety of root edge microbe. Compared with the contrast, the reclaimed water lawn irrigation was test favorable to the root microbe, especially to the bacillus. But no marked influence on the microbe of root and non-root layers. Microbial community structure and diversity were changed relatively with the irrigation of reclaimed water, which embodied the abundance increase of the dominant and sub-dominant population, non-dominant population sensitive to reclaimed water lost its original status, besides, rare population appeared or lost, the community diversity increased last.

关 键 词：再生水 微生物 种群密度 群落多样性 群落结构 草坪土壤 

分 类 号：X172[环境科学与工程—环境科学]