孕期服用叶酸预防和阻止小鼠腭裂的初步研究  

Preventive effect of folic acid in dexamethasone induced cleft palate

作　　者：黎燕[1] 孙海鹏[2] 苏放明[1] 

机构地区：[1]暨南大学第二临床医学院产科,深圳市518020 [2]暨南大学第二临床医学院口腔医学中心,深圳市518020

出　　处：《中国妇幼保健》2006年第17期2392-2393,共2页Maternal and Child Health Care of China

摘　　要：目的:探讨叶酸对地塞米松所诱导小鼠腭裂的预防和阻止作用,为唇腭裂的预防提供新的途径。方法:选取NIH系小鼠,分为5组,第1组孕鼠在GD1-15经口灌胃0·6ml的生理盐水,第2、3、4组在GD1-15分别经口灌胃10mg/kg、30mg/kg、60mg/kg的叶酸;第5组在GD13-18经口灌胃60mg/kg的叶酸。5组孕鼠均在GD12腹腔注射50mg/kg醋酸地塞米松。在GD18断颈处死孕鼠,剖腹检查胎鼠吸收和死亡情况,对活的胎鼠检查腭裂发生情况。结果:第1组未用叶酸处理的孕鼠所产胎鼠腭裂的发生率为63·8%;第2组腭裂的发生率为64·9%;第3组腭裂的发生率为58·9%;第4组腭裂发生率为42·3%;第5组腭裂的发生率为65·3%。统计学分析第4组与第1组之间差异有显著性;第2、3、5组与第1组之间差异无统计学意义。结论:只有高剂量的叶酸(60mg/kg)才能降低胎鼠腭裂的发生率;并且要从妊娠的前期开始连续使用,才能达到良好的预防效果。Objective： To explore whether the folic acid has preventive effect in dexamethasone induced cleft palate. Methods： NIH mice treated with 50 mg/kg of dexamethasone were used. Folic acid was irrigated into the animals from the 1st day to the 15th day with a dosage of 10, 30, 60 mg/kg and from the 13th day to the 18th day with a dosage of 60 mg/kg. Set up 0. 6 ml normal saline as control group. On the 18th day, pregnant females were killed by cervical dislocation. The fetuses were externally examined macroscopically and stereomicroscopically. Results： The incidence of cleft palate was decreased to 42. 3% in the 60 mg/kg group from the 1 st day to the 15th day as compared to the control value of 63. 8%. In the rest experiment groups the incidences were respectively 64. 9%, 58. 9%, 65.3%. There were no significant difference among the rest experiment groups and the control group. Conclusion： The high dosage of folic acid （60 mg/ kg） may decrease the incidence of cleft palate in dexamethasone mice during proper time.

关 键 词：先天性唇腭裂 叶酸 小鼠 胎鼠 

分 类 号：R394[医药卫生—医学遗传学]