载脂蛋白H基因多态性与冠心病的关系  

THE RELATIONSHIP BETWEEN APOLIPOPROTEIN H GENE POLYMORPHISM AND CORONARY HEART DISEASE

作　　者：王永军[1] 崔国方[1] 张典文[1] 孟竹[2] 宋达琳[2] 曲春雁[1] 

机构地区：[1]青岛市海慈医院心内科,山东青岛266033 [2]青岛市市立医院特一科

出　　处：《齐鲁医学杂志》2006年第5期399-400,403,共3页Medical Journal of Qilu

摘　　要：目的探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)的关系。方法采用聚合酶链反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病病人的ApoH外显子3、8基因型。结果冠心病组外显子3的GG基因型频率为81.8%,GA+AA基因型频率为18.2%,G等位基因频率为88.0%,A等位基因频率为12.0%,与对照组比较差异无显著性(χ2=2.147、1.454,P>0.05)。冠心病组外显子8的GG基因型频率为74.5%,GC基因型频率为25.5%,G等位基因频率为87.0%,C等位基因频率为13.0%,与对照组比较,冠心病组的GC基因型及C等位基因频率显著增高(χ2=7.742、3.907,P<0.05)。结论ApoH外显子3基因多态性与冠心病无相关性;ApoH外显子8 GC基因型及C等位基因与冠心病有关。Objective To investigate the relationship between polymorphism of apolipoprotein H （Aport） exon 3 and exon 8 with coronary heart disease （CHD）. Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of Aport exon 3 and exon 8 in 110 patients with CHD and 100 healthy adults. Results The frequency of genotype GG of exon 3 in the CHD group was 81.8%; in GA＋AA genotype, 18.2% ; in G allele, 88.0% ; in A allele, 12.0%. There was no significant difference compared with the controls X^2= 2. 147,1. 454 ; P〉0.05）. The frequency of GG genotype of exon 8 was 74.5% ; GC genotype, 25.5 % ; G allele, 87.0 %. GC genotype and C allele in C HD group were significantly higher than that in the control group X^2= 7. 742,3. 907;P〈0.05）. Conclusion The polymorphism of the apolipoprotein H exon 3 has no relationship with CHD, but the GC genotype of Aport exon 8 and C allele have close relation with CHD.

关 键 词：载脂蛋白类 外显子 多态性 单核苷酸 冠状动脉疾病 

分 类 号：R541.4[医药卫生—心血管疾病]