原发性肝细胞癌病人PBMCs中HBV X、P基因的整合  

INTEGRATION OF HBV X AND P GENES IN PBMCs IN PATIENTS WITH PRIMARY HEPATOCELLULAR CARCINOMA

作　　者：王淑霞[1] 闫志勇[2] 王力秋[1] 赵海燕[1] 刘成玉[2] 王斌[2] 

机构地区：[1]临沂市人民医院放疗科,山东临沂276003 [2]青岛大学医学院

出　　处：《青岛大学医学院学报》2006年第3期225-227,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科技发展计划项目(05-1-NS-70)

摘　　要：目的探讨外周血单个核细胞(PBMCs)中乙型肝炎病毒(HBV)X、P基因整合与原发性肝细胞癌(HCC)的关系。方法分离HBsAg阳性的40例原发性肝癌病人PBMCs,提取基因组DNA;分别设计HBV X基因、P基因PCR引物,检测HCC病人PBMCs基因组中HBV的X基因和P基因的整合情况。结果HCC病人X基因在PBMCs中的整合率为77.5%(31/40),P基因整合率为15.0%(6/40),二者差异有显著性(χ2=31.43,P<0.001)。结论PBMCs中存在HBV X、P基因的整合,X基因片段的整合及表达是HCC发生的主要因素。Objective To investigate the relationship between primary liver cancer （PLC） and integration of HBV X gene and Pgenein peripheral blood mononuclear cells （PBMCs） in PI.C patients. Methods Primers X1, X2 were designed for HBV X Gene and P1, P2 for P gene. Extracted DNA from isolated PBMCs in 40 PI.C patients with HBsAg positive. PCR was used to determine the integration of HP, VPand X gene. Results The integration of XgeneandPgenewas77.5% （31/40） and 15.0% （6/40） respectively. The difference was significant （x^2=31.43,P〈0.001）. Conclusion The integration of HBV X and P exists in peripheral blood mononuclear cells, and the expression of X gene plays a major role in the occurrence of primary liver cancer.

关 键 词：癌 肝细胞 肝炎病毒 乙型 基因 病毒整合 

分 类 号：R735.7[医药卫生—肿瘤]