二甲苯对正常人外周血淋巴细胞SCE频率的影响  

EFFECTS OF XYLENE ON SCE FREQUENCIES IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES

作　　者：刘金成[1] 苏爱[1] 

机构地区：[1]青岛大学医学院生物学教研室,山东青岛266021

出　　处：《青岛大学医学院学报》2006年第3期262-262,264,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨二甲苯对正常人外周血淋巴细胞DNA的损伤作用。方法采用染色体分析技术,对体外培养的外周血淋巴细胞姊妹染色单体交换(SCE)频率进行观察。结果3.0、6.0 mmol/L二甲苯组淋巴细胞中的SCE计数平均值分别为7.533±0.234、10.933±0.325,与对照组(2.800±0.242)比较,差异具有极显著性(t=14.084、20.085,P<0.001)。结论二甲苯对正常人外周血淋巴细胞DNA有显著损伤作用。Objective To investigate the damage of xylene on DNA of human peripheral blood lymphocytes. Methods Chromosome analysis technique was used to study sister chromatid exchange （SCE） frequencies of in-vitro-culture blood lymphocyte. Results The frequencies of SCE in xylene groups （3.0 retool/ L, 6.0 retool/ L） were 7. 533±0. 234 and 10. 933±0. 325 respectively, which were significantly higher than that of the control group （2. 800±0. 242） （t=14.084,20. 085;P〈0.001）. Conclusion The injury of xylene to human peripheral blood lymphocytes is significant.

关 键 词：二甲苯类 淋巴细胞 姐妹染色单体交换 

分 类 号：R363[医药卫生—病理学]