siRNA抑制SOM基因表达的有效序列筛选  

The Inhibition Effects of Various siRNA on Expression of Somatostatin Gene

作　　者：朱晓燕[1] 刘书漫[1] 张钦宪[1] 

机构地区：[1]郑州大学基础医学院组胚教研室,河南郑州450052

出　　处：《肿瘤基础与临床》2006年第5期356-359,共4页journal of basic and clinical oncology

基　　金：河南省重大科技攻关基金资助项目(编号:0222031300)

摘　　要：目的研究siRNA对胃癌细胞BGC-823生长抑素(SOM)分泌的抑制效应。方法设计2条针对SOM基因不同位点的寡核苷酸序列,应用RiboMAXT7体外转录合成siRNA并转染胃癌细胞系BGC-823,经RT-PCR,免疫细胞化学法检测SOM mRNA和蛋白的表达水平,筛选抑制效果最佳的序列。结果转染后24 h、48 h及72 h,SOM基因的表达被抑制,抑制效率有差异。结论体外合成siRNA可以抑制SOM基因的表达,抑制效率呈现浓度及时间依赖性。Objective To study the inhibition effects of various siRNA on expression of somatostatin in gastric cancer cell line BGC-823. Methods Two nucleotide sequences of siRNA corresponding to different sites of somatostatin gene were designed, siRNA was synthesized in vitro transcription RiboMAXT7 and transfected into gastric cancer cell line BGC-823. The inhibitory effects were detected by RT-PCR and immunohistochemical techniques to select the best sequences that inhibit SOM gene expression. MTT assay was used to determine the growth of BGC-823 cells, Results The somatostatin gene expression was suppressed for 24 h, 48 h, and 72 h after transfection, and the efficiency was different. Conclusions siRNA was synthesized in vitro and it inhibited SOM gene expression, The inhibitory effects displayed in both a time- and concentration-dependent manner.

关 键 词：生长抑素 RNA干扰 抑制 胃癌细胞系 

分 类 号：R735.2[医药卫生—肿瘤]