姜黄素对前列腺癌细胞核转录因子抑制蛋白表达的影响  

Curcumin-induced the expression of inhibitor κBα protein in human prostate cancer cells

作　　者：郭辉[1] 谌科[1] 余建华[2] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [2]武警湖北省总队医院泌尿外科

出　　处：《中华外科杂志》2006年第18期1256-1259,共4页Chinese Journal of Surgery

摘　　要：目的观察姜黄素对前列腺癌细胞核转录因子抑制蛋白(IkBα)表达的影响,探讨姜黄素抑制前列腺癌细胞增殖的作用机制。方法分别用10、25、50、75和100μmol/L 浓度的姜黄素对雄激素依赖性及雄激素非依赖性前列腺癌细胞株 LNCaP 和 PC3进行干预,5、12和24 h 后采用噻唑蓝(MTT)比色法观察细胞增殖情况;采用流式细胞术测定24 h 后细胞周期变化;5 h 后 Western 印迹法检测细胞中 IkBα的表达。结果姜黄素显著抑制 LNCaP 及 PC3细胞的生长,呈剂量和时间依赖性;姜黄素将两种前列腺癌细胞阻滞于 G_2、M 期[LNCaP 与 PC3细胞,空白对照分别为(11.4±1.3)%与(17.3±1.7)%,100μmol/L 姜黄素作用后分别为(27.3±2.8)%与(33.4±4.0)%],从而诱导肿瘤细胞凋亡;姜黄素作用于 LNCaP 细胞后,细胞中 IkBα表达无变化(F=0.129,P>0.05);但作用于 PC3细胞后,细胞中 IkBα的表达明显增强,呈现出显著的剂量依赖性(F=31.618,P<0.05)。结论姜黄素通过活化 IkBα在 PC3细胞中的表达发挥抑制 PC3细胞增殖的作用。对于 LNCaP 细胞,姜黄素可能通过抗氧化、抑制细胞内代谢产物形成等方式抑制 LNCaP 细胞增殖。Objective To investigate the curcumin-induced the expression of IκBα in androgendependent （LNCaP） and androgen-independent （PC3） prostate cancer cells, and to study the mechanisms of curcumin on the proliferative inhibition of prostate cancer cells. Methods After LNCaP and PC3 cells were affected by 10, 25, 50, 75, 100 μmol/L curcumin respectively, the cell activity was assayed with methyl thiazolyl tetrazolium （MTY） method at 5, 12 and 24 hours; Flow cytometry was adopted to observe the cell cycle of LNCaP and PC3 cells at 24 hours. After 5 hours, the expression of IκBα in LNCaP and PC3 cells was observed with Western blotting. Results Curcumin obviously suppressed the proliferation of LNCaP and PC3 cells in does-dependent and time-dependent manners. Curcumin could arrest the cell cycle of LNCaP and PC3 cells at G2 ,M phase and then induce cell apoptosis. The expression of IκBα LNCaP cells had no significant difference after using curcumin （ F = 0. 129, P 〉 0. 05 ）. However, the expression of IκBα in PC3 cells increased gradually with the inducement of concentration-increased curcumin （ F = 31. 618,P 〈0. 05）. Conclusions IκBα may play a role in the curcumin inducing apoptosis of PC3 cell, while the curcumin inducing apoptosis of LNCaP cells is by antioxidation and inhibiting metabolites formation in LNCaP cells.

关 键 词：姜黄素 前列腺肿瘤 肿瘤细胞 培养的 抑制蛋白 

分 类 号：R737.25[医药卫生—肿瘤]